https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&feed=atom&action=history
Michalak Slawomir - Revision history
2024-03-28T10:40:54Z
Revision history for this page on the wiki
MediaWiki 1.36.1
https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&diff=195368&oldid=prev
Beno Marija at 10:28, 4 March 2020
2020-03-04T10:28:08Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:28, 4 March 2020</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[Michalak Slawomir| Slawomir Michalak ]]: Visiting scientist at the [[Oroboros Laboratories: visiting scientists |Oroboros O2k-Laboratory]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[Michalak Slawomir| Slawomir Michalak ]]: Visiting scientist at the [[Oroboros Laboratories: visiting scientists |Oroboros O2k-Laboratory]]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::::* <del style="font-weight: bold; text-decoration: none;">2018, </del>January 23 to February 03</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::::* January 23 to February 03 <ins style="font-weight: bold; text-decoration: none;">2018</ins></div></td></tr>
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Beno Marija
https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&diff=194395&oldid=prev
Gnaiger Erich: Gnaiger Erich moved page Michalak S to Michalak Slawomir over redirect
2020-02-27T09:23:58Z
<p>Gnaiger Erich moved page <a href="/index.php/Michalak_S" class="mw-redirect" title="Michalak S">Michalak S</a> to <a href="/index.php/Michalak_Slawomir" title="Michalak Slawomir">Michalak Slawomir</a> over redirect</p>
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<td colspan="1" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:23, 27 February 2020</td>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&diff=188036&oldid=prev
Gnaiger Erich at 02:57, 19 November 2019
2019-11-19T02:57:13Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 02:57, 19 November 2019</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>== Visiting scientist in the Oroboros <del style="font-weight: bold; text-decoration: none;">MitoFit </del>Laboratory ==</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>== Visiting scientist in the Oroboros <ins style="font-weight: bold; text-decoration: none;">O2k-</ins>Laboratory ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: [[Image:O2k-Network.png|left|40px|link=O2k-Network|O2k-Network]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: [[Image:O2k-Network.png|left|40px|link=O2k-Network|O2k-Network]]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[Michalak Slawomir| Slawomir Michalak ]]: Visiting scientist at the [[Oroboros <del style="font-weight: bold; text-decoration: none;">MitoFit lab</del>: visiting scientists |Oroboros <del style="font-weight: bold; text-decoration: none;">MitoFit </del>Laboratory]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[Michalak Slawomir| Slawomir Michalak ]]: Visiting scientist at the [[Oroboros <ins style="font-weight: bold; text-decoration: none;">Laboratories</ins>: visiting scientists |Oroboros <ins style="font-weight: bold; text-decoration: none;">O2k-</ins>Laboratory]]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::::* January 23 to February 03 <del style="font-weight: bold; text-decoration: none;">2018</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::::* <ins style="font-weight: bold; text-decoration: none;">2018, </ins>January 23 to February 03</div></td></tr>
</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&diff=187176&oldid=prev
Beno Marija at 15:40, 30 October 2019
2019-10-30T15:40:03Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:40, 30 October 2019</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== MitoEAGLE feedback ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== MitoEAGLE feedback ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* ''I would like to participate in WG4 - MitoEAGLE data repository for blood cells and clutured cells. We are already working with cryopreserved PBMC from lung cancer, breast cancer and colon cancer patients. Thus, I would like to participate in WG4 tasks. '' - '''[[Michalak S|Slawomir Michalak]]''' (2016).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* ''I would like to participate in WG4 - MitoEAGLE data repository for blood cells and clutured cells. We are already working with cryopreserved PBMC from lung cancer, breast cancer and colon cancer patients. Thus, I would like to participate in WG4 tasks. '' - '''[[Michalak S|Slawomir Michalak]]''' (2016).</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Participated at ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Participated at ==</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">::::* [[MiP2019/MitoEAGLE Belgrade RS|MitoEAGLE 2019 Belgrade RS]]</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[ESCI 2019 Coimbra PT]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[ESCI 2019 Coimbra PT]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE_WG4_Matrei_a._Brenner_2019-03| MitoEAGLE 2019 Matrei am Brenner AT]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE_WG4_Matrei_a._Brenner_2019-03| MitoEAGLE 2019 Matrei am Brenner AT]]</div></td></tr>
</table>
Beno Marija
https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&diff=179936&oldid=prev
Beno Marija at 09:38, 3 June 2019
2019-06-03T09:38:44Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Participated at ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Participated at ==</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::* [[ESCI 2019 Coimbra PT<del style="font-weight: bold; text-decoration: none;">]]</del>]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::* [[ESCI 2019 Coimbra PT]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE_WG4_Matrei_a._Brenner_2019-03| MitoEAGLE 2019 Matrei am Brenner AT]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE_WG4_Matrei_a._Brenner_2019-03| MitoEAGLE 2019 Matrei am Brenner AT]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]</div></td></tr>
</table>
Beno Marija
https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&diff=179935&oldid=prev
Beno Marija at 09:38, 3 June 2019
2019-06-03T09:38:33Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Participated at ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Participated at ==</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">::::* [[ESCI 2019 Coimbra PT]]]]</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE_WG4_Matrei_a._Brenner_2019-03| MitoEAGLE 2019 Matrei am Brenner AT]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE_WG4_Matrei_a._Brenner_2019-03| MitoEAGLE 2019 Matrei am Brenner AT]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]</div></td></tr>
</table>
Beno Marija
https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&diff=175628&oldid=prev
Nirschl Lisa at 10:38, 20 March 2019
2019-03-20T10:38:15Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:38, 20 March 2019</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Participated at ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Participated at ==</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div> </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">::::* [[MitoEAGLE_WG4_Matrei_a._Brenner_2019-03| MitoEAGLE 2019 Matrei am Brenner AT]]</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE Lund 2018| MitoEAGLE 2018 Lund SE]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE Lund 2018| MitoEAGLE 2018 Lund SE]]</div></td></tr>
</table>
Nirschl Lisa
https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&diff=175347&oldid=prev
Gnaiger Erich at 21:31, 17 March 2019
2019-03-17T21:31:31Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 21:31, 17 March 2019</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: 1. Aim and motivation. Blood cells, like peripheral mononuclear cells (PBMCs) and platelets, can be obtained with minimal invasive procedures and used for analyzes of mitochondrial respiration for diagnostic and research purposes. Up to now I have analyzed by means of ELISA technique the expression of mitochondrial proteins in PBMCs from movement disorders and cancer patients. With the use of high-resolution respirometry I have analyzed mitochondrial respiration in PBMCs in movement disorders and cancer patients. The results were already published. Moreover, I have performed methodological study on the effects of anticoagulants on mitochondrial respiration in PBMCs. The results of the studies in movement disorders patients and on anticoagulant effects were my contributions to the Working Group 4 in COST Action MITOEAGLE. Currently, I do have the opportunity to perform analyzes of Ca+2 ions and mitochondrial membrane potential basing on tetraphenylphosphonium (TPP+) or TPMP+ evaluations using potentiometric ion-selective electrodes (ISE) and of reactive oxigen species using the system for high-resolution respirometry (HRR). However, ISE and HRR techniques require specific skills I would like to acquire during STSM I apply to and it is the aim of this STMS. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: 1. Aim and motivation. Blood cells, like peripheral mononuclear cells (PBMCs) and platelets, can be obtained with minimal invasive procedures and used for analyzes of mitochondrial respiration for diagnostic and research purposes. Up to now I have analyzed by means of ELISA technique the expression of mitochondrial proteins in PBMCs from movement disorders and cancer patients. With the use of high-resolution respirometry I have analyzed mitochondrial respiration in PBMCs in movement disorders and cancer patients. The results were already published. Moreover, I have performed methodological study on the effects of anticoagulants on mitochondrial respiration in PBMCs. The results of the studies in movement disorders patients and on anticoagulant effects were my contributions to the Working Group 4 in COST Action MITOEAGLE. Currently, I do have the opportunity to perform analyzes of Ca+2 ions and mitochondrial membrane potential basing on tetraphenylphosphonium (TPP+) or TPMP+ evaluations using potentiometric ion-selective electrodes (ISE) and of reactive oxigen species using the system for high-resolution respirometry (HRR). However, ISE and HRR techniques require specific skills I would like to acquire during STSM I apply to and it is the aim of this STMS. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: 2. Proposed contributions to the scientific objectives of the Action I will contribute the results of the studies on calcium flow, mitochondrial membrane potential and production of reactive oxigen species in PBMCs in physiological and pathological conditions to the repository of Working Group 4 in COST Action MITOEAGLE. Moreover, I will continue the studies on methodology and protocols for mitochondrial analyzes in PBMCs using new techniques I will learn during STSM. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: 2. Proposed contributions to the scientific objectives of the Action I will contribute the results of the studies on calcium flow, mitochondrial membrane potential and production of reactive oxigen species in PBMCs in physiological and pathological conditions to the repository of Working Group 4 in COST Action MITOEAGLE. Moreover, I will continue the studies on methodology and protocols for mitochondrial analyzes in PBMCs using new techniques I will learn during STSM. </div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: 3. Techniques The techniques I will learn during STSM include potentiometric analyses using ion-selective electrodes (ISE) and high-resolution <del style="font-weight: bold; text-decoration: none;">FluoRespirometry </del>by means of MultiSensor applications. My training during STSM will fully contribute to the scientific objectives of MitoEAGLE according to the Memorandum of Understanding.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: 3. Techniques The techniques I will learn during STSM include potentiometric analyses using ion-selective electrodes (ISE) and high-resolution <ins style="font-weight: bold; text-decoration: none;">respirometry </ins>by means of MultiSensor applications. My training during STSM will fully contribute to the scientific objectives of MitoEAGLE according to the Memorandum of Understanding.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&diff=175285&oldid=prev
Gnaiger Erich at 19:22, 17 March 2019
2019-03-17T19:22:37Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 19:22, 17 March 2019</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== MitoEAGLE Short-Term Scientific Mission ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== MitoEAGLE Short-Term Scientific Mission ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>****: [[Short-Term Scientific Missions MitoEAGLE#STSM_Grant_Period_2 |STSM Grant Period 2]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>****: [[Short-Term Scientific Missions MitoEAGLE#STSM_Grant_Period_2 |STSM Grant Period 2]]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::: '''Work <del style="font-weight: bold; text-decoration: none;">Plan </del>summary'''</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::: '''Work <ins style="font-weight: bold; text-decoration: none;">plan </ins>summary'''</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>:::: 1. Aim and motivation. Blood cells, like peripheral mononuclear cells (PBMCs) and platelets, can be obtained with minimal invasive procedures and used for analyzes of mitochondrial respiration for diagnostic and research purposes. Up to now I have analyzed by means of ELISA technique the expression of mitochondrial proteins in PBMCs from movement disorders and cancer patients. With the use of high-resolution respirometry I have analyzed mitochondrial respiration in PBMCs in movement disorders and cancer patients. The results were already published. Moreover, I have performed methodological study on the effects of anticoagulants on mitochondrial respiration in PBMCs. The results of the studies in movement disorders patients and on anticoagulant effects were my contributions to the Working Group 4 in COST Action MITOEAGLE. Currently, I do have the opportunity to perform analyzes of Ca+2 ions and mitochondrial membrane potential basing on tetraphenylphosphonium (TPP+) or TPMP+ evaluations using potentiometric ion-selective electrodes (ISE) and of reactive oxigen species using the system for <del style="font-weight: bold; text-decoration: none;">High</del>-<del style="font-weight: bold; text-decoration: none;">Resolution FluoRespirometry </del>(<del style="font-weight: bold; text-decoration: none;">HRFR</del>). However, ISE and <del style="font-weight: bold; text-decoration: none;">HRFR </del>techniques require specific skills I would like to acquire during STSM I apply to and it is the aim of this STMS. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::: 1. Aim and motivation. Blood cells, like peripheral mononuclear cells (PBMCs) and platelets, can be obtained with minimal invasive procedures and used for analyzes of mitochondrial respiration for diagnostic and research purposes. Up to now I have analyzed by means of ELISA technique the expression of mitochondrial proteins in PBMCs from movement disorders and cancer patients. With the use of high-resolution respirometry I have analyzed mitochondrial respiration in PBMCs in movement disorders and cancer patients. The results were already published. Moreover, I have performed methodological study on the effects of anticoagulants on mitochondrial respiration in PBMCs. The results of the studies in movement disorders patients and on anticoagulant effects were my contributions to the Working Group 4 in COST Action MITOEAGLE. Currently, I do have the opportunity to perform analyzes of Ca+2 ions and mitochondrial membrane potential basing on tetraphenylphosphonium (TPP+) or TPMP+ evaluations using potentiometric ion-selective electrodes (ISE) and of reactive oxigen species using the system for <ins style="font-weight: bold; text-decoration: none;">high</ins>-<ins style="font-weight: bold; text-decoration: none;">resolution respirometry </ins>(<ins style="font-weight: bold; text-decoration: none;">HRR</ins>). However, ISE and <ins style="font-weight: bold; text-decoration: none;">HRR </ins>techniques require specific skills I would like to acquire during STSM I apply to and it is the aim of this STMS. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: 2. Proposed contributions to the scientific objectives of the Action I will contribute the results of the studies on calcium flow, mitochondrial membrane potential and production of reactive oxigen species in PBMCs in physiological and pathological conditions to the repository of Working Group 4 in COST Action MITOEAGLE. Moreover, I will continue the studies on methodology and protocols for mitochondrial analyzes in PBMCs using new techniques I will learn during STSM. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: 2. Proposed contributions to the scientific objectives of the Action I will contribute the results of the studies on calcium flow, mitochondrial membrane potential and production of reactive oxigen species in PBMCs in physiological and pathological conditions to the repository of Working Group 4 in COST Action MITOEAGLE. Moreover, I will continue the studies on methodology and protocols for mitochondrial analyzes in PBMCs using new techniques I will learn during STSM. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: 3. Techniques The techniques I will learn during STSM include potentiometric analyses using ion-selective electrodes (ISE) and high-resolution FluoRespirometry by means of MultiSensor applications. My training during STSM will fully contribute to the scientific objectives of MitoEAGLE according to the Memorandum of Understanding.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: 3. Techniques The techniques I will learn during STSM include potentiometric analyses using ion-selective electrodes (ISE) and high-resolution FluoRespirometry by means of MultiSensor applications. My training during STSM will fully contribute to the scientific objectives of MitoEAGLE according to the Memorandum of Understanding.</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>== MitoEAGLE <del style="font-weight: bold; text-decoration: none;">Feedback </del>==</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>== MitoEAGLE <ins style="font-weight: bold; text-decoration: none;">feedback </ins>==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* ''I would like to participate in WG4 - MitoEAGLE data repository for blood cells and clutured cells. We are already working with cryopreserved PBMC from lung cancer, breast cancer and colon cancer patients. Thus, I would like to participate in WG4 tasks. '' - '''[[Michalak S|Slawomir Michalak]]''' (2016).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* ''I would like to participate in WG4 - MitoEAGLE data repository for blood cells and clutured cells. We are already working with cryopreserved PBMC from lung cancer, breast cancer and colon cancer patients. Thus, I would like to participate in WG4 tasks. '' - '''[[Michalak S|Slawomir Michalak]]''' (2016).</div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Michalak_Slawomir&diff=172622&oldid=prev
Nirschl Lisa at 14:48, 11 February 2019
2019-02-11T14:48:15Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:48, 11 February 2019</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::*[[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE Lund 2018| MitoEAGLE 2018 Lund SE]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE Lund 2018| MitoEAGLE 2018 Lund SE]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE Poznan 2018|MitoEAGLE 2018 Poznan PL]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::* [[MitoEAGLE Poznan 2018|MitoEAGLE 2018 Poznan PL]]</div></td></tr>
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Nirschl Lisa