Difference between revisions of "MiPNet25.03 IOC144 Innsbruck AT"
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|Welcome - Get-together: Introduction of participants and their research interests | |Welcome - Get-together: Introduction of participants and their research interests | ||
| | |Oroboros MitoFit Laboratory | ||
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|08:30 | |08:30 | ||
|OroboPOS service and O2k instrumental setup - overview with videoclips | |OroboPOS service and O2k instrumental setup - overview with videoclips | ||
| | |Oroboros MitoFit Laboratory | ||
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|09:15 | |09:15 | ||
|'''Hands-on''': OroboPOS service and O2k instrumental setup | |'''Hands-on''': OroboPOS service and O2k instrumental setup | ||
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|09:55 | |09:55 | ||
|DatLab 7.4 overview | |DatLab 7.4 overview | ||
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|10:30 | |10:30 | ||
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|Instrumental quality control 1: oxygen calibration | |Instrumental quality control 1: oxygen calibration | ||
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|11:30 | |11:30 | ||
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DL-Protocol: O2 calibration air | DL-Protocol: O2 calibration air | ||
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|'''Hands-on''': Instrumental quality control 1: oxygen calibration (continuation) | |'''Hands-on''': Instrumental quality control 1: oxygen calibration (continuation) | ||
DL-Protocol: O2 calibration air | DL-Protocol: O2 calibration air | ||
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|13:30 | |13:30 | ||
|Instrumental quality control 2: Instrumental O<sub>2</sub> background – overview with videoclips | |Instrumental quality control 2: Instrumental O<sub>2</sub> background – overview with videoclips | ||
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|14:00 | |14:00 | ||
|'''Hands-on''':Instrumental quality control 2: Instrumental O<sub>2</sub> background | |'''Hands-on''':Instrumental quality control 2: Instrumental O<sub>2</sub> background | ||
DL-Protocol: Instrumental O2 background TiP2k | DL-Protocol: Instrumental O2 background TiP2k | ||
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|15:00 | |15:00 | ||
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|15:30-17:30 | |15:30-17:30 | ||
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis. | |'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis. | ||
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DL-Protocol: O2 calibration air | DL-Protocol: O2 calibration air | ||
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|09:15 | |09:15 | ||
|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 | |Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 | ||
| | |Oroboros MitoFit Laboratory | ||
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|09:45 | |09:45 | ||
|SUITbrowser: how to find the best SUIT protocol for your research questions. | |SUITbrowser: how to find the best SUIT protocol for your research questions. | ||
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|10:00 | |10:00 | ||
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DL-Protocol: SUIT-002 O2 ce-pce D007.DLP | DL-Protocol: SUIT-002 O2 ce-pce D007.DLP | ||
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|11:45 | |11:45 | ||
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DL-Protocol: O2k-cleaning AfterUse | DL-Protocol: O2k-cleaning AfterUse | ||
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DL-Protocol: O2k-cleaning AfterUse | DL-Protocol: O2k-cleaning AfterUse | ||
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|13:30 | |13:30 | ||
|DatLab 7.4 analysis: Introduction and new features | |DatLab 7.4 analysis: Introduction and new features | ||
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|15:00 | |15:00 | ||
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|15:30-17:30 | |15:30-17:30 | ||
|Individual DatLab analysis + summary | |Individual DatLab analysis + summary | ||
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DL-Protocol: O2 calibration air | DL-Protocol: O2 calibration air | ||
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|'''MitoFit proficiency test | |'''MitoFit proficiency test''' | ||
'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002). | '''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002). | ||
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DL-Protocol: SUIT-002 O2 ce-pce D007.DLP | DL-Protocol: SUIT-002 O2 ce-pce D007.DLP | ||
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DL-Protocol: O2k-cleaning AfterUse | DL-Protocol: O2k-cleaning AfterUse | ||
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|13:15 | |13:15 | ||
|DatLab analysis | |DatLab analysis | ||
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|14:00 | |14:00 | ||
|O2k-Applications - overview | |O2k-Applications - overview | ||
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|14:30 | |14:30 | ||
|Proficiency test: DatLab analysis summary | |Proficiency test: DatLab analysis summary | ||
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|15:00 | |15:00 |
Revision as of 10:46, 6 November 2019
MiPNet25.03 IOC144 Innsbruck AT
Innsbruck AT, 2020 Jan 20-22. MitoFit Coaching Days - Basic IOC144. |
Oroboros (2020-01-20) Mitochondr Physiol Network
Abstract:
MitoFit Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 20-22.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- MitoFit Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the Coaching Days.
Venue
- » Oroboros MitoFit Laboratory
- Schoepfstrasse 18, 6020 Innsbruck
- » Information on travel and venue: IOC Innsbruck.
- » Hotel recommendation: Basic Hotel Innsbruck***(https://www.basic-hotel.at/en/)
- Using code "OROBOROS" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.
- Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2€ City tax to be charged additionally.
- » Oroboros MitoFit Laboratory
Registration
- Download registration form, fill in the pdf and then print to sign: download pdf
Tutors and lecturers
Luiza Cardoso, PhD., Oroboros Instruments - PostDoc
Marco Di Marcello, BSc., Oroboros Instruments - Scientific Assistant
Carolina Doerrier, PhD. - Chief scientific officer
Luiz Felipe Garcia-Souza, PhD. student, Oroboros Instruments
Erich Gnaiger, PhD., Oroboros Instruments, CEO
Preliminary programme
Monday 20th
Topic Room 08:15 Welcome - Get-together: Introduction of participants and their research interests Oroboros MitoFit Laboratory 08:30 OroboPOS service and O2k instrumental setup - overview with videoclips Oroboros MitoFit Laboratory 09:15 Hands-on: OroboPOS service and O2k instrumental setup Oroboros MitoFit Laboratory 09:55 DatLab 7.4 overview Oroboros MitoFit Laboratory 10:30 Coffee 11:00 Instrumental quality control 1: oxygen calibration Oroboros MitoFit Laboratory 11:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
Oroboros MitoFit Laboratory 12:00 Lunch break 13:00 Hands-on: Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air
Oroboros MitoFit Laboratory 13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips Oroboros MitoFit Laboratory 14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background DL-Protocol: Instrumental O2 background TiP2k
Oroboros MitoFit Laboratory 15:00 Coffee 15:30-17:30 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. Oroboros MitoFit Laboratory
Tuesday 21st
Topic Room 08:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
Oroboros MitoFit Laboratory 09:15 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 Oroboros MitoFit Laboratory 09:45 SUITbrowser: how to find the best SUIT protocol for your research questions. Oroboros MitoFit Laboratory 10:00 Coffee 10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002). DL-Protocol: SUIT-001 O2 ce-pce D003.DLP
DL-Protocol: SUIT-002 O2 ce-pce D007.DLP
Oroboros MitoFit Laboratory 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse
Oroboros MitoFit Laboratory 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use (continuation) DL-Protocol: O2k-cleaning AfterUse
Oroboros MitoFit Laboratory 13:30 DatLab 7.4 analysis: Introduction and new features Oroboros MitoFit Laboratory 15:00 Coffee 15:30-17:30 Individual DatLab analysis + summary Oroboros MitoFit Laboratory
Wednesday 22nd
Topic Room 08:30 MitoFit proficiency test Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
Oroboros MitoFit Laboratory 10:00 Coffee 10:30 MitoFit proficiency test Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).
DL-Protocol: SUIT-001 O2 ce-pce D003.DLP
DL-Protocol: SUIT-002 O2 ce-pce D007.DLP
Oroboros MitoFit Laboratory 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse
Oroboros MitoFit Laboratory 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use (continuation) DL-Protocol: O2k-cleaning AfterUse
Oroboros MitoFit Laboratory 13:15 DatLab analysis Oroboros MitoFit Laboratory 14:00 O2k-Applications - overview Oroboros MitoFit Laboratory 14:30 Proficiency test: DatLab analysis summary Oroboros MitoFit Laboratory 15:00 Coffee 15:30 The Bioblast wiki, MitoFit Preprint Archives, O2k-Network and MitoEAGLE MiPArt 16:30 Feedback & conclusions MiPArt 17:00 Farewell activity MiPArt
Participants
Participant | Institution | |
---|---|---|
Buoso Chiara | AT Innsbruck Weiss G - Medical University Innsbruck, Innsbruck (AT) | |
Griffiths Kate | UK Glasgow Selman C - Glasgow University, Glasgow (UK) |
Contact
- Oroboros Instruments GmbH
- High-Resolution Respirometry
- Schoepfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- E-Mail: [email protected]
- Mitochondria and Cell Research
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
ORO, IOC, MitoGlobal, Next, 2020