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MiPNet25.03 IOC144 Innsbruck AT

Innsbruck AT, 2020 Jan 20-22. MitoFit Coaching Days - Basic IOC144.

Oroboros (2020-01-20) Mitochondr Physiol Network

Abstract:

MitoFit Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 20-22.

• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit

General information

MitoFit Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the Coaching Days.

Venue

» Oroboros MitoFit Laboratory

Schoepfstrasse 18, 6020 Innsbruck

» Information on travel and venue: IOC Innsbruck.

» Hotel recommendation: Basic Hotel Innsbruck***(https://www.basic-hotel.at/en/)

Using code "OROBOROS" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.

Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2€ City tax to be charged additionally.

Registration

Download registration form, fill in the pdf and then print to sign: download pdf

Tutors and lecturers

Luiza Cardoso, PhD., Oroboros Instruments - PostDoc
Marco Di Marcello, BSc., Oroboros Instruments - Scientific Assistant
Carolina Doerrier, PhD. - Chief scientific officer
Luiz Felipe Garcia-Souza, PhD. student, Oroboros Instruments
Erich Gnaiger, PhD., Oroboros Instruments, CEO

Preliminary programme

Monday 20th

	Topic	Room
08:15	Welcome - Get-together: Introduction of participants and their research interests	ORO-MitoFit Lab
08:30	OroboPOS service and O2k instrumental setup - overview with videoclips	ORO-MitoFit Lab
09:15	Hands-on: OroboPOS service and O2k instrumental setup	ORO-MitoFit Lab
09:55	DatLab 7.4 overview	ORO-MitoFit Lab
10:30	Coffee
11:00	Instrumental quality control 1: oxygen calibration	ORO-MitoFit Lab
11:30	Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	ORO-MitoFit Lab
12:00	Lunch break
13:00	Hands-on: Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air	ORO-MitoFit Lab
13:30	Instrumental quality control 2: Instrumental O₂ background – overview with videoclips	ORO-MitoFit Lab
14:00	Hands-on:Instrumental quality control 2: Instrumental O₂ background DL-Protocol: Instrumental O2 background TiP2k	ORO-MitoFit Lab
15:00	Coffee
15:30-17:30	Hands-on: Instrumental quality control 1 and 2. DatLab analysis.	ORO-MitoFit Lab

Tuesday 21st

	Topic	Room
08:30	Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	ORO-MitoFit Lab
09:15	Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2	ORO-MitoFit Lab
09:45	SUITbrowser: how to find the best SUIT protocol for your research questions.	ORO-MitoFit Lab
10:00	Coffee
10:30	Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002). DL-Protocol: SUIT-001 O2 ce-pce D003.DLP DL-Protocol: SUIT-002 O2 ce-pce D007.DLP	ORO-MitoFit Lab
11:45	Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse	ORO-MitoFit Lab
12:00	Lunch break
13:00	Hands-on: O2k-cleaning after use (continuation) DL-Protocol: O2k-cleaning AfterUse	ORO-MitoFit Lab
13:30	DatLab 7.4 analysis: Introduction and new features	ORO-MitoFit Lab
15:00	Coffee
15:30-17:30	Individual DatLab analysis + summary	ORO-MitoFit Lab

Wednesday 22nd

	Topic	Room
08:30	MitoFit proficiency test Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	ORO-MitoFit Lab
10:00	Coffee
10:30	MitoFit proficiency test: Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002). DL-Protocol: SUIT-001 O2 ce-pce D003.DLP DL-Protocol: SUIT-002 O2 ce-pce D007.DLP	ORO-MitoFit Lab
11:45	Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse	ORO-MitoFit Lab
12:00	Lunch break
13:00	Hands-on: O2k-cleaning after use (continuation) DL-Protocol: O2k-cleaning AfterUse	ORO-MitoFit Lab
13:15	DatLab analysis	ORO-MitoFit Lab
14:00	O2k-Applications - overview	ORO-MitoFit Lab
14:30	Proficiency test: DatLab analysis summary	ORO-MitoFit Lab
15:00	Coffee
15:30	The Bioblast wiki, MitoFit Preprint Archives, O2k-Network and MitoEAGLE	MiPArt
16:30	Feedback & conclusions	MiPArt
17:00	Farewell activity	MiPArt

Participants

	Participant	Institution
	Buoso Chiara	AT Innsbruck Weiss G - Medical University Innsbruck, Innsbruck (AT)
	Griffiths Kate	UK Glasgow Selman C - Glasgow University, Glasgow (UK)

Contact

Oroboros Instruments GmbH

High-Resolution Respirometry

Schoepfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

E-Mail: [email protected]

Mitochondria and Cell Research

IOC recommended reading

» IOC recommended reading

» MitoPedia: Respiratory states

O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.

Labels:

ORO, IOC, MitoGlobal, Next, 2020

@@ Line 68: / Line 68: @@
 |ORO-MitoFit Lab
 |-
-|09:30
+|09:15
 |'''Hands-on''': OroboPOS service and O2k instrumental setup
 |ORO-MitoFit Lab
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-|10:10
+|09:55
-|'''Hands-on''': DatLab 7
+|DatLab 7.4 overview
 |ORO-MitoFit Lab
 |-
@@ Line 87: / Line 87: @@
 |'''Hands-on''': Instrumental quality control 1: oxygen calibration
-DL-Protocol: O2k-cleaning before use
+DL-Protocol: O2k-cleaning BeforeUse
 DL-Protocol: O2 calibration air
@@ Line 97: / Line 97: @@
 |-
 |13:00
-|'''Hands-on''':Instrumental quality control 1: oxygen calibration (continuation)
+|'''Hands-on''': Instrumental quality control 1: oxygen calibration (continuation)
 DL-Protocol: O2 calibration air
 |ORO-MitoFit Lab
 |-
 |13:30
-|Instrumental quality control 2: Instrumental O2 background – overview with videoclips
+|Instrumental quality control 2: Instrumental O<sub>2</sub> background – overview with videoclips
 |ORO-MitoFit Lab
 |-
 |14:00
-|'''Hands-on''':Instrumental quality control 2: Instrumental O2 background
+|'''Hands-on''':Instrumental quality control 2: Instrumental O<sub>2</sub> background
 DL-Protocol: Instrumental O2 background TiP2k
 |ORO-MitoFit Lab
@@ Line 114: / Line 114: @@
 |
 |-
-|15:30-18:00
+|15:30-17:30
 |'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
 |ORO-MitoFit Lab
@@ Line 134: / Line 134: @@
 |style="width: 15em"|'''Room'''
 |-
-|09:00
+|08:30
 |Hands-on: Instrumental quality control 1: oxygen calibration
-DL-Protocol: O2k-cleaning before use
+DL-Protocol: O2k-cleaning BeforeUse
 DL-Protocol: O2 calibration air
@@ Line 143: / Line 143: @@
 |-
 |09:15
-|Application of the O2k Series H
+|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2
 |ORO-MitoFit Lab
 |-
-|09:30
+|09:45
-|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2
+|SUITbrowser: how to find the best SUIT protocol for your research questions.
 |ORO-MitoFit Lab
 |-
@@ Line 155: / Line 155: @@
 |-
 |10:30
-|'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
+|'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).
-DL-Protocol: SUIT-1_O2_pce_D03.DLP
+DL-Protocol: SUIT-001 O2 ce-pce D003.DLP
-DL-Protocol: SUIT-2 O2 pce D07.DLP
+DL-Protocol: SUIT-002 O2 ce-pce D007.DLP
 |ORO-MitoFit Lab
@@ Line 166: / Line 166: @@
 |'''Hands-on''': O2k-cleaning after use
-DL-Protocol: O2k-cleaning after use
+DL-Protocol: O2k-cleaning AfterUse
 |ORO-MitoFit Lab
 |-
@@ Line 174: / Line 174: @@
 |-
 |13:00
-|'''Hands-on''': O2k-cleaning after use
+|'''Hands-on''': O2k-cleaning after use (continuation)
-DL-Protocol: O2k-cleaning after use
+DL-Protocol: O2k-cleaning AfterUse
 |ORO-MitoFit Lab
 |-
 |13:30
-|Introduction to DatLab analysis
+|DatLab 7.4 analysis: Introduction and new features
 |ORO-MitoFit Lab
 |-
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 |
 |-
-|15:30-18:00
+|15:30-17:30
 |Individual DatLab analysis + summary
 |ORO-MitoFit Lab
@@ Line 206: / Line 206: @@
 |style="width: 15em"|'''Room'''
 |-
-|09:00
+|08:30
-|'''MitoFit proficiency test:'''
+|'''MitoFit proficiency test'''
 '''Hands-on''': Instrumental quality control 1: oxygen calibration
-DL-Protocol: O2k-cleaning before use
+DL-Protocol: O2k-cleaning BeforeUse
 DL-Protocol: O2 calibration air
@@ Line 224: / Line 224: @@
 |'''MitoFit proficiency test:'''
-'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
+'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).
-DL-Protocol: SUIT-1_O2_pce_D03.DLP
+DL-Protocol: SUIT-001 O2 ce-pce D003.DLP
-DL-Protocol: SUIT-2 O2 pce D07.DLP
+DL-Protocol: SUIT-002 O2 ce-pce D007.DLP
 |ORO-MitoFit Lab
@@ Line 235: / Line 235: @@
 |'''Hands-on''': O2k-cleaning after use
-DL-Protocol: O2k-cleaning after use
+DL-Protocol: O2k-cleaning AfterUse
 |ORO-MitoFit Lab
 |-
@@ Line 243: / Line 243: @@
 |-
 |13:00
-|'''Hands-on''': O2k-cleaning after use
+|'''Hands-on''': O2k-cleaning after use (continuation)
-DL-Protocol: O2k-cleaning after use
+DL-Protocol: O2k-cleaning AfterUse
 |ORO-MitoFit Lab
 |-
@@ Line 253: / Line 253: @@
 |-
 |14:00
-|DatLab analysis summary
+|O2k-Applications - overview
+|ORO-MitoFit Lab
+|-
+|14:30
+|Proficiency test: DatLab analysis summary
 |ORO-MitoFit Lab
 |-
@@ Line 261: / Line 265: @@
 |-
 |15:30
-|The Bioblast wiki, O2k-Network and MITOEAGLE
+|The Bioblast wiki, MitoFit Preprint Archives, O2k-Network and MitoEAGLE
 |MiP''Art''
 |-
-|16:15
+|16:30
 |Feedback & conclusions
 |MiP''Art''