Difference between revisions of "MiPNet21.06 SUIT RP"

Revision as of 09:08, 19 January 2016

SUIT reference assay for OXPHOS analysis by high-resolution respirometry. - »Bioblast pdf«

» Versions

OROBOROS (2016-01-19) Mitochondr Physiol Network

Abstract: Doerrier C, Sumbalova Z, Lamberti G, Krumschnabel G, Gnaiger (2016) SUIT reference assay for OXPHOS analysis by high-resolution respirometry. Mitochondr Physiol Network 21.06(pre02):1-8.

» Instrument: O2k, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels: MiParea: Instruments;methods

Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria

Regulation: Substrate Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.

HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

O2k-SOP

Laboratory sheets for SUIT protocols RP1 and RP2

SUIT reference assay for permeabilized fibres, Pfi

Type	SUIT protocol	Sample	pdf file	docx file
Titration sheet	RP1	Pfi	»SUIT_RP1	»SUIT_RP1
Titration sheet	RP2	Pfi	»SUIT_RP2	»SUIT_RP2
Tick boxes	Combined	Pfi	»RP print	»RP print

Pre-publication communication

This communication is a pre-publication, inviting critical feedback and comments. All feedback will be carefully documented and evaluated in terms of justification of co-authorship. We intend to finally publish this topic in a peer-reviewed journal as an original article, with reference to the pre-publication history including reviewer's reports. - Gnaiger Erich 03:23, 18 January 2016 (CET)

Further details

Introduction » Gnaiger 2014 MitoPathways
Table of titrations » MiPNet09.12 O2k-Titrations
Definition » Substrate-uncoupler-inhibitor titration
Context » SUIT protocol library
Abbreviations » MitoPedia

@@ Line 26: / Line 26: @@
 |-
 ! Type
-! Protocol
+! SUIT protocol
+! Sample
 ! pdf file
 ! docx file
 |-
 | Titration sheet
-| RP1-Pfi
+| RP1
-| [[Media:SUIT_RP1-Pfi.pdf |»SUIT_RP1-Pfi]]
+| Pfi
-| [[Media:SUIT_RP1-Pfi.docx |»SUIT_RP1-Pfi]]
+| [[Media:SUIT_RP1-Pfi.pdf |»SUIT_RP1]]
+| [[Media:SUIT_RP1-Pfi.docx |»SUIT_RP1]]
 |-
 | Titration sheet
-| RP2-Pfi
+| RP2
-| [[Media:SUIT_RP2-Pfi.pdf |»SUIT_RP2-Pfi]]
+| Pfi
-| [[Media:SUIT_RP2-Pfi.docx |»SUIT_RP2-Pfi]]
+| [[Media:SUIT_RP2-Pfi.pdf |»SUIT_RP2]]
+| [[Media:SUIT_RP2-Pfi.docx |»SUIT_RP2]]
 |-
 | Tick boxes
-| RP-Pfi
+| Combined
-| [[Media:RP-Pfi print.pdf |»RP-Pfi print]]
+| Pfi
-| [[Media:RP-Pfi print.docx |»RP-Pfi print]]
+| [[Media:RP-Pfi print.pdf |»RP print]]
+| [[Media:RP-Pfi print.docx |»RP print]]
 |}