View source for MiPNet18.13 IOC84 Alaska

{{OROBOROS header page name}}
{{Publication
|title=[[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]] 
'''Big Lake, Alaska US''', 2014 Jan 04-07. Oroboros O2k-Workshop on HRR and [[MiPNet18.13 IOC84 Alaska|Racing dogs at Iditarod]], '''IOC84'''.  »[[US OK Stillwater Davis MS]]
|info=[[Miller 2017 PLOS ONE]]
|authors=OROBOROS
|year=2014-01-04
|journal=Mitochondr Physiol Network
|abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]]
'''84th Oroboros O2k-Workshop on high-resolution respirometry ''' in cooperation with O2k-Network Lab [[US OK Stillwater Davis MS]].
 Project: Racing dogs at Iditarod, Alaska 2014 with integrated O2k-Workshop

|mipnetlab=US OK Stillwater Davis MS, AT Innsbruck OROBOROS
}}
[[Image:The world as a laboratory.jpg|right|180px|link=http://wiki.oroboros.at/index.php/The_world_as_a_laboratory|Science and adventure]]
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== IOC84: Racing dogs at Iditarod, Alaska 2014 ==
::::A special expertise of the Oroboros mitoscience-team is the logistic planning and support of expeditions with a focus on mitochondrial physiology. '''IOC84''' was integrated in the Alasca expedition as a training course.
::::>> [[MiPNet18.13 IOC84 Alaska|IOC84]]
::::* Collaboration of [[Davis MS|'''Michael Davis''']] and '''Katherine Williamson''' ([[US OK Stillwater Davis MS|Comparative Exercise Physiology Laboratory, Oklahoma State University]]) with [http://www.buserdog.com '''Martin Buser'''] (Happy Trails Kennel, Alaska), [[Boushel RC|'''Robert Boushel''']] ([[SE Stockholm Boushel RC|Swedish School of Sport and Health Sciences, Stockholm, SE]]), '''Benjamin F. Miller''' and '''Karyn Hamilton''' (Colorado State University, US), and '''Erich Gnaiger''' and [[Laner V|'''Verena Laner''']] ([[AT_Innsbruck_Gnaiger E|Medical University of Innsbruck]] and [[AT_Innsbruck_OROBOROS|Oroboros Instruments]]) - a [[Power-O2k]] project with six Oroboros O2k.

== Results published ==
::::Data generated in this collaborative study are published: [[Miller 2017 PLOS ONE]].

== Lecturers and tutors ==

::::[[Davis Michael S]], Professor, Physiological Sciences Oxley Equine Professor 
::::Director, Comparative Exercise Physiology Lab 

::::[[File:Gnaiger Erich.jpg|80px|Erich Gnaiger]]
::::[[Gnaiger Erich]], CEO, Oroboros Instruments

::::[[File:VG.jpg|80px|Laner Verena]]
::::[[Laner Verena]]; Project manager and application specialist, Oroboros INSTRUMENTS

::::[[File:Boushel.jpg|80px|Boushel Robert C]]
::::[[Boushel RC]]; Professor and Chair in Physiology, The Swedish School of Sport and Health Sciences


::::Alaska Expedition pre-study ("Iditarod"). Collaboration with Michael Davis (Oklahoma State University, US), Robert Boushel (Swedish School of Sport and Health Sciences, Stockholm, SE) and Benjamin F. Miller (Colorado State University, US) - with integrated IOC84 (Jan 04-07).


::::Dr. Erich Gnaiger (Medical University of Innsbruck and Oroboros Instruments) and his collaborator Verena Laner (Oroboros Instruments) joined the project of  [[Davis MS|Michael Davis]] (Oklahoma State University) in Big Lake, Alaska. Cumulative training effects on the mitochondrial function of alaskan sled dogs were studied in January and March 2014 at Happy Trails Kennel in Big Lake, Alaska. Further participants were [[Boushel RC|Robert Boushel]] (Institute for Sport and Health Sciences, Stockholm, SE) as well as Benjamin F. Miller and Karyn Hamilton (Colorado State University, Fort Collins, US).


== Racing dogs at Iditarod, Alaska 2014 ==

::::Iditarod - The last great race(r) 2014: the greatest challenge up to the finish: » [http://iditarod.com iditarod.com]


::::At Martin Buser's 'Happy Trails Kennel' (Big Lake, near Anchorage, Alaska) Prof. Michael Davis (Comparative Exercise Physiology Laboratory, Oklahoma State University, USA) conducts a long-term collaboration with Martin's team to study and train the Alaskan dogs in preparation of long-distance races, the most challenging of which is the 1,100 mile Iditarod race. Martin Buser is 4 times champion of the Iditarod, with a scientifically oriented interest in the study of the genetics and physiology of the dogs that goes far beyond the race.

::::The line of mixed-breed Alaskan huskies of Martin incorporates sprint-dog lines and is selected and trained as much for long-distance endurance as for speed - and happiness. "Happy dogs perform better and make racing more fun" (Martin Buser).

::::» [http://buserdog.com buserdog.com]

::::In January and March 2014, Mike Davis and Katherine Williamson teamed up with Erich Gnaiger and Verena Laner (Medical University of Innsbruck and Oroboros Instruments), Robert Boushel (Swedish School of Sport and Health Sciences, Stockholm, SE), and Benjamin F. Miller and Karyn Hamilton (Colorado State University, US) to focus on mitochondrial respiratory function in the skeletal muscle of the dogs. With a Power-O2k approach using six Oroboros O2k, these mitochondrial studies are conducted at the beginning of the intensive training period and after the Iditarod race, considered as the final stage of the most intensive endurance training.

::::» [[Gnaiger 2014 MitoPathways |Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis.]]


<gallery mode=packed widths="192px" heights="165px" perrow=3>
File:Iditarod1.jpg|The Iditarod team of fast endurance dogs
File:Iditarod2.jpg|Martin Buser (Jan 2014)
File:Iditarod3.jpg|'Heavy reading' on a light sled (musher Martin Buser)
</gallery>


== The tutors==
<gallery mode=packed widths="192px" heights="165px" perrow=5>
File:VerenaLaner.jpg|Verena Laner and Robert Boushel titrating simultaneously into multiple chambers of the Power-O2k array, seeing the muscle fibres through the windows of the O2k-Chambers, while we see the dogs in the kennel through the lab window.
File:Robert.jpg|Robert Boushel at muscle fibre preparation with the new Oroboros cooling plate. The snow for cooling BIOPS and MiR06Cr is readily available outside.
</gallery>
== The science team ==


<gallery mode=packed widths="192px" heights="165px" perrow=5>
File:Teampic.jpg|The IOC84 team at Happy Trails Kennel, left to right, back row: Verena Laner, Karyn Hamilton, Robert Boushel, Katherine Williamson, Michael Davis, Shannon Massie; front row: Erich Gnaiger, Benjamin F. Miller.
File:Iditarod4.jpg|Left to right, back: Karyn Hamilton, Benjamin F. Miller, Robert Boushel, Katherine Chapoton, Michael Davis, Katherine Williamson, Verena Laner; front: Shannon Massie, Martin Buser, Erich Gnaiger.
File:Iditarod5.jpg|Left to right: Shannon Massie, Verena Laner, Karyn Hamilton, Benjamin F. Miller, Katherine Williamson, Michael Davis, Katherine Chapoton, Martin Buser, Erich Gnaiger, Robert Boushel.
File:Iditarod6.jpg|The Mito-Science-Team (left to right: Rob Boushel, Erich Gnaiger, Karyn Hamilton, Verena Laner and Benjamin F. Miller) working in the lab. (C) by Shannon Massie.
File:Iditarod7.jpg|Getting ready for an exciting sled-ride through the Alaskan nature - Rob Boushel and Rohn Buser.
</gallery>

== Impressions of the study ==

=== The fast endurance heroes ===

<gallery mode=packed widths="192px" heights="165px" perrow=3>
File:Hund1.jpg|on
File:Hund2.jpg|the
File:Hund3.jpg|treadmill
File:Hund4.jpg
File:Hund5.jpg
File:Hund6.jpg
File:Hund7.JPG
File:Hund8.JPG
File:Hund9.jpg
</gallery>


=== The mitochondrial lab at Happy Trails Kennel ===

::::Dog skeletal muscle mitochondria: the first detailed study with SUIT protocols.

<gallery mode=packed widths="192px" heights="165px" perrow=5>
File:Power02k.jpg|A Power-O2k approach with six Oxygraph-2k in Pelicases and the microbalance in the transport case
File:I1.JPG|Katherine Williamson
File:Expedition Alaska.jpg|Erich Gnaiger
File:I3.jpg|Verena Laner and dog 'Lucky'
File:I4.jpg|Verena Laner, Robert Boushel
</gallery>


=== Getting connected ===

<gallery mode=packed widths="192px" heights="165px" perrow=5>
File:Aa1.jpg
File:A2.jpg
File:A3.jpg
</gallery>

===What do the dogs tell us?===
<gallery mode=packed widths="192px" heights="165px" perrow=5>
File:Dog.jpg
File:Dog1.jpg
File:Dog2.jpg
File:Dog3.jpg
</gallery>

=== A visitor at Happy Trails Kennel ===

<gallery mode=packed widths="192px" heights="165px" perrow=5>
File:A4.jpg| Moose - not yet studied in terms of mitochondrial physiology
</gallery>

=== The mito team ===

::::* [[US_OK_Stillwater_Davis_MS| O2k-Network Lab: US OK Stillwater Davis MS]]
::::* [[SE_Stockholm_Boushel_RC| O2k-Network Lab: SE Stockholm Boushel RC]]
::::* [[AT_Innsbruck_Gnaiger_E| O2k-Network Lab: AT Innsbruck Gnaiger E]]
::::* [[Gnaiger_E| Gnaiger Erich]]
::::* [[Laner_V| Laner Verena]]

<gallery mode=packed widths="192px" heights="165px" perrow=5>
File:B1.jpg|Setting up the mito lab with Martin Buser
File:B2.jpg|Katherine Chapoton, Katherine Williamson and Mike Davis discuss the races.
File:B3.jpg|Mike Davis and Erich Gnaiger discuss the [[MitoPedia:_SUIT#SUIT_protocols |SUIT protocols]].
</gallery>


=== Alaska Expedition main study (March 2014) ===
::::* Trained cohort
::::: SUIT protocols [[SUIT_FNS(PGM)03]], [[SUIT_NS(PGM)04]]
<gallery mode=packed widths="192px" heights="165px" perrow=5>
File:A1.jpg
File:Aa2.jpg
File:Aa3.jpg
File:Aa4.jpg
File:A5.jpg
File:A6.jpg
File:A7.jpg
File:A8.jpg
File:A9.jpg
</gallery>

===Titrations for SUIT protocols===
<gallery mode=packed widths="192px" heights="165px" perrow=5>
File:Titration for SUIT.jpg|Left to right: Shannon Massie, Benjamin F. Miller, Erich Gnaiger, Karyn Hamilton, Robert Boushel
File:Titration for SUIT-benjamin.jpg|Left to right:Benjamin F. Miller, Erich Gnaiger, Karyn Hamilton, Shannon Massie, Robert Boushel
</gallery>

===Permeabilized fibre preparation===
<gallery mode=packed widths="192px" heights="165px" perrow=5>
File:Karyn.jpg|Karyn Hamilton: only 1.4 to 1.8 mg wet weight were prepared for each O2k-Chamber.
File:Testing.jpg|Testing the new Oroboros cooling plate.
</gallery>

::::Fotos by Gnaiger Erich and Laner Verena


== Further links of interest ==

::::* [https://www.youtube.com/watch?v=jj7EhZC6lqY Youtube]
::::* [http://www.k300.org k300]: Congratulations to Rohn Buser for winning this race. "Rohn won it in 38 h and change, but not without a MAJOR challenge from Jeff King who made a run at Rohn in the last 5-10 miles, but couldn't overtake him. 300 miles (500 km) in 38 h. When you subtract the 10 h of mandatory rest, that means Rohn's dogs AVERAGED nearly 18 kph for over a day. These are the dogs we will investigate in March. That isn't even the race record. Ironically, the race record of 37 h 4 min is held by Martin Buser" (Michael S. Davis, 2014-01-20).


== Recommended reading ==
::::* Miller B, Hamilton K, Boushel R, Williamson K, Laner V, Gnaiger E, Davis M (2017) Mitochondrial respiration in highly aerobic canines in the non-raced state and after a 1600-km sled dog race. PLOS ONE 12:e0174874. - [[Miller 2017 PLOS ONE |»Bioblast link«]]

::: O2k-Core Manual
::::* [[Media:MiPNet19.01 O2k-Core Manual.pdf|'''»O2k-Core Manual.pdf''']]

::: SUIT protocols for high-resolution respirometry
::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]]
::::* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |»Bioblast Access]]

::: Mitochondrial pathways
::::* Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp. - [[Gnaiger 2014 MitoPathways |»Bioblast link«]] '''''handout to O2k-Workshop participants'''''.

[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] 
:::: O2k-Workshops are listed as [[MitoGlobal Events]].
::::* This workshop is a component of the K-Regio project ''[[MitoCom_O2k-Fluorometer|MitoCom]]''.
:::: [[Image:MitoCom Tyrol.jpg|right|150px|link=http://wiki.oroboros.at/index.php/Welcome_to_MitoCom|MitoCom]]
:::: [[File:K-Regio Icon Englisch 01.jpg||right|250px]]

{{Labeling
|instruments=Oxygraph-2k
|additional=ORO, IOC, 2014, O2k-Network, MitoCom
}}



[[Category:O2k-Workshops]]