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Difference between revisions of "MiPNet18.10 O2k-Specifications"

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{{OROBOROS header page name}}
{{Publication
{{Publication
|title=Gnaiger E, Fasching M (2014) Oxygraph-2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10.
|title=[[Image:Logo OROBOROS INSTRUMENTS.jpg|right|60px|link=http://www.oroboros.at|Oroboros ]] O2k-specifications for respirometry and comprehensive OXPHOS analysis.
|info=[[Media:MiPNet18.10 O2kvsMultiwell.pdf|'''Open Access-MiPNet18.10.pdf''']] ; [http://www.bioblast.at/index.php/File:MiPNet18.10_O2kvsMultiwell.pdf Versions]
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/d/db/MiPNet18.10_O2k_specifications.pdf|Bioblast pdf]] Β» [http://www.bioblast.at/index.php/File:MiPNet18.10_O2k_specifications.pdf Versions]
|authors=OROBOROS
|authors=Oroboros
|year=2014
|year=2019-09-19
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|keywords=O2k-Specifications
|abstract=Gnaiger E (2019) O2k-specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(09):1-8.
|mipnetlab=AT Innsbruck OROBOROS
{{MiPNet pdf page linking to MitoPedia}}
|mipnetlab=AT Innsbruck Oroboros
}}
}}
== Further information ==
::::Β» [[MiPNet06.05 Test Experiments on O2k-Specifications]]
::::Β» [[Comparison of respirometric methods]]
<br />
'''Acknowledgements'''
[[File:Template NextGen-O2k.jpg|left|400px|link=NextGen-O2k]]
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{{Labeling
{{Labeling
|area=Respiration, Instruments;methods
|area=Respiration, Instruments;methods
|organism=Human
|organism=Human
|model cell lines=Fibroblast
|tissues=Fibroblast
|preparations=Intact cells, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated Mitochondria, SMP
|preparations=Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Intact cells
|instruments=Oxygraph-2k, TIP2k, Fluorometry, pH, NO, TPP, Ca, Theory
|instruments=Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, Theory
}}
|additional=Comparison of respirometric methods, MitoFit2022QC
>> '''''More details on O2k-Specifications?'' [http://www.oroboros.at/?O2k-Specifications Consult OROBOROS]'''
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== Discussion ==
Scientists who have experience with both instruments are invited to extend the discussion:
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::>> [[Talk:MiPNet18.10_O2k_versus_multiwell]]
::>> [[Talk:Rogers 2011 PLoS One|XFe for isolated mitochondria - a critical discussion]]
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== Selected related links ==
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::>> [http://www.oroboros.at/?low-o2k-running-costs Low running costs for the OROBOROS O2k]
::>> [http://www.oroboros.at/?Power-O2k Power-O2k]
:: >> [[O2k-Publications:_Specific_topics#Tissues_and_cell_types]]
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== Conflict of interest ==
:: [[Gnaiger E|Erich Gnaiger]] is founder and CEO of OROBOROS INSTRUMENTS, and responsible for the development of the Oxygraph-2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations.
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== Selected references ==
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::* [[Gnaiger 1995 J Bioenerg Biomembr|Gnaiger E, Steinlechner-Maran R, MΓ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.]]
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::* [[Steinlechner-Maran 1996 Am J Physiol Cell Physiol|Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271: C2053-C2061.]]
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::* [[Gnaiger 2000 Proc Natl Acad Sci U S A|Gnaiger E, MΓ©ndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.]]
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::* [[Gnaiger 2001 Respir Physiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]]
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::* [[Huetter 2004 Biochem J|HΓΌtter E, Renner K, Pfister G, StΓΆckl P, Jansen-DΓΌrr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380: 919-928.]]
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::* [[Kuznetsov 2004 Am J Physiol Heart Circ Physiol|Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–H1641.]]
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::* [[Gnaiger 2008 POS|Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.]]
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::* [[Scandurra 2010 Adv Exp Med Biol|Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25.]]
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::* [[Aguirre 2010 Biochim Biophys Acta|Aguirre E, RodrΓ­guez-JuΓ‘rez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797: 557-565.]]
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::* [[Votion 2012 PLoS One|Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.]]
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::* [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]
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::* [[Gnaiger 2012 MitoPathways|Gnaiger E (2012) Mitochondrial Pathways and Respiratory Control. An Introduction to OXPHOS Analysis. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp.]]
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{{MitoPedia methods
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|mitopedia method=Respirometry
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|type=Respiration
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}}
}}
{{MitoPedia topics|type=Respiration
}}
[[Category:OroboPedia]]
[[Category:OroboPedia]]

Revision as of 09:29, 5 May 2022

                



MiPNet18.10 O2k-Specifications

Publications in the MiPMap
Oroboros
O2k-specifications for respirometry and comprehensive OXPHOS analysis.

Β» Bioblast pdf Β» Versions

Oroboros (2019-09-19) Mitochondr Physiol Network

Abstract: Gnaiger E (2019) O2k-specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(09):1-8.

O2k-technical support and open innovation
Open the pdf document above.
Β» Current O2k-series: NextGen-O2k Series XB and O2k Series J
Β» Current software versions DatLab 8.0: MitoPedia: DatLab


β€’ O2k-Network Lab: AT Innsbruck Oroboros

Further information

Β» MiPNet06.05 Test Experiments on O2k-Specifications
Β» Comparison of respirometric methods


Acknowledgements

Template NextGen-O2k.jpg







Labels: MiParea: Respiration, Instruments;methods 


Organism: Human  Tissue;cell: Fibroblast  Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Intact cells 



HRR: Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, Theory 

Comparison of respirometric methods, MitoFit2022QC