Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "MiPNet17.17 Amplex-Mouse-brain"

From Bioblast
Β 
(11 intermediate revisions by 5 users not shown)
Line 1: Line 1:
{{OROBOROS header page name}}
{{Publication
{{Publication
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</sub> production in mouse brain mitochondria.
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</sub> production in mouse brain mitochondria.
|info=[[Media:MiPNet17.17_Amplex-Mouse-brain.pdf|'''Open Access-MiPNet17.17.pdf''']] ;[http://www.bioblast.at/index.php/File:MiPNet17.17_Amplex-Mouse-brain.pdf Versions]
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/e/e2/MiPNet17.17_Amplex-Mouse-brain.pdf|Bioblast pdf]] Β»[http://www.bioblast.at/index.php/File:MiPNet17.17_Amplex-Mouse-brain.pdf Versions]
|authors=OROBOROS
|authors=Oroboros
|year=2014-11-14
|year=2014-11-14
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract='''Fasching M, Sumbalova Z, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</sub> production in mouse brain mitochondria. Mitochondr Physiol Network 17.17.'''
|abstract=Fasching M, Sumbalova Z, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</sub> production in mouse brain mitochondria. Mitochondr Physiol Network 17.17.
{{MiPNet pdf page linking to MitoPedia}}


[[High-resolution respirometry]] [1] is extended by MultiSensor techniques allowing the simultaneous measurement of oxygen consumption and one additional parameter (mitochondrial membrane potential, pH, Ca<sup>2+</sup>, or NO concentration [2-5]). Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels.
:Β» Product:[[O2k-Fluo_LED2-Module | O2k-Fluo LED2-Module]]
Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
Β 
# Quality control of preparations by respirometric performance.
|mipnetlab=AT_Innsbruck_Oroboros, AT Innsbruck MitoCom
# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores.
# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
# Additional information is acquired for a limited amount of sample.
# Oxygen dependence of parameters can be studied.
Here we introduce the O2k-Fluorescence LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H<sub>2</sub>O<sub>2</sub> production of mouse brain mitochondria.
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
|mipnetlab=AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom
}}
{{Labeling
|area=Respiration, Instruments;methods
|organism=Mouse
|tissues=Nervous system
|preparations=Isolated mitochondria
|topics=Uncoupler
|couplingstates=LEAK, OXPHOS, ETS
|substratestates=CI, CII, CIII, ROX
|instruments=Oxygraph-2k, O2k-Fluorometer, Protocol
|additional=O2k-Demo, O2k-MultiSensor
}}
}}
== Further Information ==
'''References'''
# [[O2k-Publications: O2k-Fluorometry]]
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]
# [[Aguirre 2010 Biochim Biophys Acta|
Aguirre E, RodrΓ­guez-JuΓ‘rez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797: 557-565.]]
# [http://www.oroboros.at/index.php?mipnetanalyzer MiPNetAnalyzer]
# [[Hickey_2012_J_Comp_Physiol_B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182: 91-100.]]
# [[Krumschnabel_2014_Methods_Mol_Biol| Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2014) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol [in press].]]
# [[Gnaiger 2012 MitoPathways|Gnaiger E (2012) Mitochondrial Pathways and Respiratory Control. An Introduction to OXPHOS Analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp.]]

Latest revision as of 09:54, 27 November 2019

                



MiPNet17.17 Amplex-Mouse-brain

Publications in the MiPMap
O2k-Protocols
O2k-Fluorometry: HRR and H2O2 production in mouse brain mitochondria.

Β» Bioblast pdf Β»Versions

Oroboros (2014-11-14) Mitochondr Physiol Network

Abstract: Fasching M, Sumbalova Z, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse brain mitochondria. Mitochondr Physiol Network 17.17.

O2k-technical support and open innovation
Open the pdf document above.
Β» Current O2k-series: NextGen-O2k Series XB and O2k Series J
Β» Current software versions DatLab 8.0: MitoPedia: DatLab
Β» Product: O2k-Fluo LED2-Module


β€’ O2k-Network Lab: AT_Innsbruck_Oroboros, AT Innsbruck MitoCom