MiPNet17.04 CitrateSynthase

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Eigentler A, Draxl A, Wiethüchter A, Kuznetsov AV, Lassnig B, Gnaiger E. Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr Physiol Network 17.04.

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OROBOROS (

) Mitochondr Physiol Network

Abstract: O2k-Protocol for Citrate Synthase

Citrate synthase (E.c. 4.1.3.7) is a pace-maker enzyme in the Krebs cycle (citric acid cycle). Citrate synthase, CS, has a molecular weight of 51,709 Da, with gene map locus 12q13.2-q13.3. CS is localized in the mitochondrial matrix, but is nuclear encoded, synthesized on cytoplasmic ribosomes and transported into the mitochondrial matrix. CS, therefore, is commonly used as a quantitative marker enzyme for the content of intact mitochondria (Holloszy et al 1970; Willimas et al 1986; Hood et al 1989), although this role of CS has been questioned in developmental (Drahota et al 2004), age-related studies (Marin-Garcia et al 1998), physical training (Pesta et al 2011) and cardiac disease studies (Lemieux et al 2011). Proliferation of mitochondria in pathological states is sometimes associated with an increase in CS activity per cell, but CS activity in a specific tissue is frequently constant when expressed per mitochondrial protein or per mt-respiratory capacity (Renner et al 2003). Mitochondrial, cellular or tissue respiration, therefore, may be expressed per CS activity for specific applications (Kuznetsov et al 2002; Renner et al 2003; Hütter el al 2004).

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O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods, mt-Biogenesis;mt-density 



Preparation: Enzyme  Enzyme: Marker Enzyme"Marker Enzyme" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. 


HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 



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