Difference between revisions of "MiPNet10.04 CellRespiration"

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MiPNet10.04 CellRespiration

An experiment with high-resolution respirometry: coupling control in cell respiration.

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Oroboros (2016-08-08) Mitochondr Physiol Network

Abstract: Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12.

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Labels: MiParea: Respiration, Instruments;methods

Organism: Mouse Tissue;cell: Blood cells Preparation: Intact cells

Regulation: Coupling efficiency;uncoupling, Uncoupler Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k, TIP2k, O2k-Protocol

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+{{OROBOROS header page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] An experiment with high-resolution respirometry: Phosphorylation control in cell respiration.
-|info=[[Media:MiPNet10.04 CellRespiration.pdf|'''Open Access-MiPNet10.04.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions]
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] An experiment with high-resolution respirometry: coupling control in cell respiration.
-|authors=OROBOROS
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/a/a6/MiPNet10.04_CellRespiration.pdf |Bioblast pdf]]  »[http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions]
-|year=2014-02
+|authors=Oroboros
+|year=2016-08-08
 |journal=Mitochondr Physiol Network
-|abstract='''Gnaiger E (2014) An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04(07): 1-12.'''
+|abstract=Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12.
-Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual  [http://bioblast.at/images/e/ef/MiPNet09.12_O2k-Titrations.pdf titrations] of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump [http://www.bioblast.at/images/8/8e/MiPNet04.05_Titration-Injection.pdf TIP2k ]. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and [http://www.bioblast.at/images/2/28/MiPNet12.09_O2FluxAnalysis.pdf on-line data analysis ]. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).
-:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
+{{MiPNet pdf page linking to MitoPedia}}
-|mipnetlab=AT_Innsbruck_OROBOROS}}
+:» Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]]
+|mipnetlab=AT Innsbruck Oroboros
+}}
 {{Labeling
 |area=Respiration, Instruments;methods
@@ Line 16: / Line 22: @@
 |preparations=Intact cells
 |topics=Coupling efficiency;uncoupling, Uncoupler
-|couplingstates=LEAK, ROUTINE, ETS
+|couplingstates=LEAK, ROUTINE, ET
-|substratestates=ROX
+|pathways=ROX
-|instruments=Oxygraph-2k, TIP2k, Protocol
+|instruments=Oxygraph-2k, TIP2k, O2k-Protocol
-|additional=O2k-Demo, O2k-Core}}
+}}
-[[Image:MiPNet10.04.jpg|center|width=80px|frame|Figure 1. Respiration of 32D cells (1.1106 ml-1). Traces for the two chambers of the Oxygraph-2k are superimposed. A: Oxygen flow [pmol O2s-1106]; B: Oxygen concentration [μM] (graph layout “7 Gr1-Flux Gr2-O2 Conc”). After inhibition of ATP synthase with oligomycin, FCCP was titrated at 0.5 μM steps with the TIP2k. Each titration step is automatically marked by an event (vertical lines). After the aerobic-anoxic transition, the chambers were opened for re-oxygenation, causing a 10-min disturbance of the traces of respiratory flow. The TIP2k needles were removed before opening, and re-inserted after closing the chamber.]]
-== Further information ==
-:>> [http://www.oroboros.at/?protocols_cell_pcp  Excel Templates and DatLab-Demo Files]
-* [[Gnaiger 2012 MitoPathways References|References]]
-[[Category:OroboPedia]]