Difference between revisions of "MiPNet10.04 CellRespiration"

Latest revision as of 12:51, 4 December 2019

MiPNet10.04 CellRespiration

An experiment with high-resolution respirometry: coupling control in cell respiration.

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Oroboros (2016-08-08) Mitochondr Physiol Network

Abstract: Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12.

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• O2k-Network Lab: AT Innsbruck Oroboros

Labels: MiParea: Respiration, Instruments;methods

Organism: Mouse Tissue;cell: Blood cells Preparation: Intact cells

Regulation: Coupling efficiency;uncoupling, Uncoupler Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k, TIP2k, O2k-Protocol

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+{{OROBOROS header page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] An experiment with high-resolution respirometry: Coupling control in cell respiration.
-|info=[[File:PDF.jpg|50px|link=http://wiki.oroboros.at/images/a/a6/MiPNet10.04_CellRespiration.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions]
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] An experiment with high-resolution respirometry: coupling control in cell respiration.
-|authors=OROBOROS
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/a/a6/MiPNet10.04_CellRespiration.pdf |Bioblast pdf]]  »[http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions]
-|year=2015-01-11
+|authors=Oroboros
+|year=2016-08-08
 |journal=Mitochondr Physiol Network
-|abstract=Gnaiger E (2015) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(08):1-12.
+|abstract=Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12.
+{{MiPNet pdf page linking to MitoPedia}}
+:» Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]]
-Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS O2k. The experiment demonstrates manual  [[MiPNet09.12 O2k-Titrations |titrations]] of inhibitors, and automatic titrations of an uncoupler using the electronic [[O2k-Catalogue: TIP2k |Titration-Injection microPump TIP2k]]. Application of the DatLab software is shown for instrumental control (O2k and TIP2k), and [[MiPNet19.18E O2 Flux Analysis |real-time data analysis]]. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the O2k Manual. The experiments were carried out by participants of an O2k-Workshop on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).
+|mipnetlab=AT Innsbruck Oroboros
-:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS O2k], [[OROBOROS O2k-Catalogue | O2k-Catalogue]]
-|mipnetlab=AT Innsbruck OROBOROS
 }}
 {{Labeling
@@ Line 17: / Line 22: @@
 |preparations=Intact cells
 |topics=Coupling efficiency;uncoupling, Uncoupler
-|couplingstates=LEAK, ROUTINE, ETS
+|couplingstates=LEAK, ROUTINE, ET
-|substratestates=ROX
+|pathways=ROX
 |instruments=Oxygraph-2k, TIP2k, O2k-Protocol
-|additional=O2k-Demo, O2k-Core
 }}
-[[Image:MiPNet10.04.jpg|center|width=80px|frame|Figure 1. Respiration of 32D cells (1.1106 ml-1). Traces for the two chambers of the O2k are superimposed. A: Oxygen flow [pmol O<sub>2</sub>·s<sup>-1</sup>·10<sup>6</sup>]; B: Oxygen concentration [μM] (graph layout “7 Gr1-Flux Gr2-O2 Conc”). After inhibition of ATP synthase with oligomycin, FCCP was titrated at 0.5 μM steps with the TIP2k. Each titration step is automatically marked by an event (vertical lines). After the aerobic-anoxic transition, the chambers were opened for re-oxygenation, causing a 10-min disturbance of the traces of respiratory flow. The TIP2k needles were removed before opening, and re-inserted after closing the chamber.]]
-== Further information ==
-:>> [http://www.oroboros.at/?protocols_cell_pcp  Excel Templates and DatLab-Demo Files]
-* [[Gnaiger 2014 MitoPathways|References]]
-* [[Coupling control protocol]]
-[[Category:OroboPedia]]