Difference between revisions of "MiPNet09.12 O2k-Titrations"

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|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/e/ef/MiPNet09.12_O2k-Titrations.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet09.12_O2k-Titrations.pdf Versions]
 
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/e/ef/MiPNet09.12_O2k-Titrations.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet09.12_O2k-Titrations.pdf Versions]
 
|authors=Oroboros
 
|authors=Oroboros
|year=2018-10-01
+
|year=2019-09-13
 
|journal=Mitochondr Physiol Network
 
|journal=Mitochondr Physiol Network
|abstract='''Oroboros (2018) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(17): 1-2.'''   
+
|abstract='''Oroboros (2019) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(18): 1-2.'''   
  
 
A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 mL O2k-chamber, and syringes used for manual titrations.
 
A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 mL O2k-chamber, and syringes used for manual titrations.

Latest revision as of 11:33, 13 September 2019

Publications in the MiPMap
O2k-Protocols
O2k manual titrations: SUIT protocols with mitochondrial preparations.

» Bioblast pdf »Versions

Oroboros (2019-09-13) Mitochondr Physiol Network

Abstract: Oroboros (2019) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(18): 1-2.

A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 mL O2k-chamber, and syringes used for manual titrations.

» O2k-Protocols
» Product: Oroboros O2k, O2k-Catalogue


O2k-Network Lab: AT_Innsbruck_Oroboros


Labels: MiParea: Instruments;methods 




Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid 


HRR: O2k-Protocol 

O2k-chemicals and media 


Note: Titration of chemicals in respirometry

Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.


Further information

» MitoPedia: SUIT - Substrate-uncoupler-inhibitor-titration (SUIT) protocols for respirometry
» MiPNet03.02 - Chemicals and media for respirometry
» MiPNet19.14 - SOP for manual O2k-titrations with Hamilton microsyringes.