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MiPNet06.06 Chemical O2 background

From Bioblast
Publications in the MiPMap
Kuznetsov AV, Gnaiger E (2001) Oxygraph assay of cytochrome c oxidase activity: Chemical background correction. Mitochondr Physiol Network 06.06.

Β» File:MiPNet06.06 ChemicalBackground.pdf; Versions

O2k-Protocols contents

OROBOROS (2001) Mitochondr Physiol Network

Abstract: O2k-Protocol for chemical background correction

Oxygraphic determination of cytochrome c oxidase activity in the presence of TMPD, ascorbate and cytochrome c requires consideration of chemical background oxygen consumption. Several compounds are readily oxidized by molecular oxygen when dissolved in water. This leads to a significant chemical oxygen consumption in the absence of any respiring biological sample. The rate of this autoxidation represents a chemical background which strongly depends on experimental conditions, such as temperature, chemical composition, pH and oxygen concentration. Moreover, the rate of autoxidation may be significantly catalyzed by metal traces and metal-containing proteins (e.g. cytochrome c). Therefore, high-resolution respirometric measurement of oxygen flux ultimately depends on chemical background correction analyzed under experimental conditions over the entire oxygen range.

>> O2k-Protocols:Overall contents
>> Product: OROBOROS Oxygraph-2k, O2k-Catalogue


β€’ O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods 



Preparation: Enzyme, Oxidase; Biochemical Oxidation"Oxidase; Biochemical Oxidation" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 



HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

O2k-SOP 


Further information

>> MiPNet06.06
  • Version 7: 2010-08-23
  • Version 1: 2001