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Difference between revisions of "MiPNet06.06 Chemical O2 background"

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{{Template:OROBOROS support page name}}
{{Publication
{{Publication
|title=Kuznetsov AV, Gnaiger E (2001) Oxygraph assay of cytochrome c oxidase activity: Chemical background correction. Mitochondr Physiol Network 06.06.
|title=[[Image:O2k-Manual.jpg|right|70px|link=O2k-Manual|O2k-Manual]] Oxygraph assay of cytochrome ''c'' oxidase activity: chemical background correction.
|info=[[File:MiPNet06.06 ChemicalBackground.pdf|'''Open Access-MiPNet06.06''']]; [http://www.bioblast.at/index.php/File:MiPNet06.06_ChemicalBackground.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|frame|O2k-Protocols contents]]
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/1/1b/MiPNet06.06_ChemicalO2Background.pdf |Bioblast pdf]] Β» [http://www.bioblast.at/index.php/File:MiPNet06.06_ChemicalO2Background.pdf Versions]
|authors=OROBOROS
|authors=Oroboros
|year=2001
|year=2015-04-27
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract='''O2k-Protocol for chemical background correction '''
|abstract=Kuznetsov AV, Gnaiger E (2010) Oxygraph assay of cytochrome ''c'' oxidase activity: chemical background correction. Mitochondr Physiol Network 06.06(07):1-4.
:Β» Product: O2k-Catalogue: [[O2k-MultiSensor]], [[O2k-Core]], [[O2k-Catalogue]]


Oxygraphic determination of cytochrome c oxidase activity in the presence of TMPD, ascorbate and cytochrome c requires consideration of chemical background oxygen consumption. Several compounds are readily oxidized by molecular oxygen when dissolved in water. This leads to a significant chemical oxygen consumption in the absence of any respiring biological sample. The rate of this autoxidation represents a chemical background which strongly depends on experimental conditions, such as temperature, chemical composition, pH and oxygen concentration. Moreover, the rate of autoxidation may be significantly catalyzed by metal traces and metal-containing proteins (e.g. cytochrome c). Therefore, high-resolution respirometric measurement of oxygen flux ultimately depends on chemical background correction analyzed under experimental conditions over the entire oxygen range.
|mipnetlab=AT_Innsbruck_Oroboros
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:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
Β 
|mipnetlab=AT_Innsbruck_OROBOROS
}}
}}
{{Labeling
{{Labeling
|area=Instruments;methods
|area=Instruments;methods
|preparations=Enzyme, Oxidase; Biochemical Oxidation
|preparations=Enzyme, Oxidase;biochemical oxidation
|instruments=Oxygraph-2k, Protocol
|enzymes=Complex IV;cytochrome c oxidase
|instruments=Oxygraph-2k, O2k-Manual
|additional=O2k-SOP
|additional=O2k-SOP
}}
}}
== Further information ==
:>> [http://www.oroboros.at/?chemical_background MiPNet06.06]
* Version 7: 2010-08-23
* Version 1: 2001
[[Category:OroboPedia]]

Latest revision as of 21:19, 13 February 2020


                  


O2k-Open Support

MiPNet06.06 Chemical O2 background


Publications in the MiPMap
O2k-Manual
Oxygraph assay of cytochrome c oxidase activity: chemical background correction.

Β» Bioblast pdf Β» Versions

Oroboros (2015-04-27) Mitochondr Physiol Network

Abstract: Kuznetsov AV, Gnaiger E (2010) Oxygraph assay of cytochrome c oxidase activity: chemical background correction. Mitochondr Physiol Network 06.06(07):1-4.

Β» Product: O2k-Catalogue: O2k-MultiSensor, O2k-Core, O2k-Catalogue


β€’ O2k-Network Lab: AT_Innsbruck_Oroboros


Labels: MiParea: Instruments;methods 



Preparation: Enzyme, Oxidase;biochemical oxidation  Enzyme: Complex IV;cytochrome c oxidase 


HRR: Oxygraph-2k, O2k-Manual 

O2k-SOP