Messer 2000 J Biomed Materials Res

Messer RLW, Doeller JE, Kraus DW, Lucas LC (2000) An investigation of fibroblast mitochondria enzyme activity and respiration in response to metallic ions released from dental alloys. J. Biomed. Materials Res. 50: 598-604.

» PMID: 10756319

Messer RLW, Doeller JE, Kraus DW, Lucas LC (2000) J. Biomed. Materials Res.

Abstract: Most cellular functions evaluated for biocompatibility are high-energy processes such as proliferation and therefore are not usually affected before a decrease in energy production is observed. Several studies have shown that metabolic functions are altered at much lower concentrations than several normally used biocompatibility tests such as viability. Therefore, the purpose of this study was to provide an in-depth evaluation of metallic ion effects on mitochondria function and thereby biocompatibility. These studies evaluated the mitochondrial function of human gingival fibroblasts exposed to the salt solutions of ions released from nickel-based dental alloys, particularly beryllium (Be(2+)), chromium (Cr(6+) and Cr(3+)), nickel (Ni(2+)), and molybdenum (Mo(6+)). Mitochondrial function was examined by NADH:CoQ reductase activity, succinate dehydrogenase activity, and oxygen consumption.

• O2k-Network Lab: US AL Birmingham Kraus DW

Labels:

Organism: Human  Tissue;cell: Fibroblast  Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 

HRR: Oxygraph-2k 

Pharmacology; Biotechnology