Menegollo 2019 Methods Mol Biol
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| Menegollo Michela, Tessari Isabella, Bubacco Luigi, Szabadkai Gyorgy (2019) Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography in Cultured Cells. Methods Mol Biol 1925:223-32. |
Menegollo Michela, Tessari Isabella, Bubacco Luigi, Szabadkai Gyorgy (2019) Methods Mol Biol
Abstract: Cytoplasmic and mitochondrial Ca2+ signals couple cellular ATP production to activity-related energy demand. In order to accurately determine the bioenergetic effect of Ca2+ signals, cellular energy charge, i.e., the compound ratio of the phosphorylated adenine nucleotides AMP, ADP, and ATP, should be estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we describe a protocol applied in our laboratories to quantify ATP, ADP, and AMP nucleotides in cellular extracts.
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