Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Manfredi 2002 Methods

From Bioblast
Publications in the MiPMap
Manfredi Giovanni, Yang Lichuan, Gajewski Carl D, Mattiazzi Marina (2002) Measurements of ATP in mammalian cells. Methods 26(4):317-26.

Β» PMID: 12054922

Manfredi Giovanni, Yang Lichuan, Gajewski Carl D, Mattiazzi Marina (2002) Methods

Abstract: Levels of phosphorylated adenosine nucleotides, including the universal energy carrier adenosine 5(')-triphosphate (ATP) and its metabolites adenosine 5(')-diphosphate (ADP) and adenosine 5(')-monophosphate (AMP), define the energy state in living cells and are dependent mainly on mitochondrial function. In this article, we describe a method based on the luciferase-luciferin system used to measure mitochondrial ATP synthesis continuously in permeabilized mammalian cells and mitochondria isolated from animal tissues. We also describe a technique that uses the expression of recombinant targeted luciferase to report ATP content in different cell compartments. Finally, we describe an HPLC-based method for accurate measurement of ATP, ADP, and AMP in cultured cells and animal tissues.

β€’ Bioblast editor: Cardoso Luiza HD


Cited by

  • Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001


Labels:






MitoFit 2021 MgG