Difference between revisions of "Manfredi 2002 Methods"
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{{Publication | {{Publication | ||
|title=Manfredi G, Yang L, Gajewski CD, Mattiazzi M (2002) Measurements of ATP in mammalian cells. Methods 26 | |title=Manfredi G, Yang L, Gajewski CD, Mattiazzi M (2002) Measurements of ATP in mammalian cells. Methods 26:317-26. | ||
|info=[[PMID: 12054922]] | |info=[[PMID: 12054922]] | ||
|authors=Manfredi Giovanni, Yang Lichuan, Gajewski Carl D, Mattiazzi Marina | |authors=Manfredi Giovanni, Yang Lichuan, Gajewski Carl D, Mattiazzi Marina | ||
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|editor=[[Cardoso Luiza HD]] | |editor=[[Cardoso Luiza HD]] | ||
}} | }} | ||
== Cited by == | == Cited by == | ||
{{Template:Cited by Cardoso 2021 MitoFit MgG}} | {{Template:Cited by Cardoso 2021 MitoFit MgG}} | ||
Latest revision as of 11:44, 9 February 2022
| Manfredi G, Yang L, Gajewski CD, Mattiazzi M (2002) Measurements of ATP in mammalian cells. Methods 26:317-26. |
Manfredi Giovanni, Yang Lichuan, Gajewski Carl D, Mattiazzi Marina (2002) Methods
Abstract: Levels of phosphorylated adenosine nucleotides, including the universal energy carrier adenosine 5(')-triphosphate (ATP) and its metabolites adenosine 5(')-diphosphate (ADP) and adenosine 5(')-monophosphate (AMP), define the energy state in living cells and are dependent mainly on mitochondrial function. In this article, we describe a method based on the luciferase-luciferin system used to measure mitochondrial ATP synthesis continuously in permeabilized mammalian cells and mitochondria isolated from animal tissues. We also describe a technique that uses the expression of recombinant targeted luciferase to report ATP content in different cell compartments. Finally, we describe an HPLC-based method for accurate measurement of ATP, ADP, and AMP in cultured cells and animal tissues.
• Bioblast editor: Cardoso Luiza HD
Cited by
- Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001
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MitoFit 2021 MgG-PW
