Malonate

high-resolution terminology - matching measurements at high-resolution

Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).

Abbreviation: Mna

Reference: MiPNet09.12

Labels:

Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. 

HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Application in HRR

Mna: Malonate (Malonic acid, CH₂(COOH)₂); Sigma M 1296, 5 g, store at -20 °C: FW = 800)

Caution: Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.

Preparation of 1 mM stock solution (dissolved in Ethanol):

1) weigh 1.27 mg of FCCP directly into a 5 ml glass vial

2) add 5 ml EtOH

3) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution

4) store in glass vials (recommendation) at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

• Titration volume: 1 µl using a 10 µl syringe
• Final conc. in 2 ml O2k-chamber: 2 µg/ml