Difference between revisions of "Malonate"

Revision as of 14:18, 22 November 2010

high-resolution terminology - matching measurements at high-resolution

Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).

Abbreviation: Mna

Labels:

Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.

HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

Application in HRR

Mna: Malonate (Malonic acid, CH₂(COOH)₂); Sigma M 1296, 5 g, store at -20 °C: FW = 800)

Caution: Very toxic. Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.

Preparation of 4 mg/ml stock solution (dissolved in H₂0):

1) weigh 4 mg of Malonic acid directly into a 2 ml glass vial.

2) add 1 ml H₂0

3) divide into 0.2 ml portions

4) store frozen at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

Titration volume: 1 µl using a 10 µl syringe
Final conc. in 2 ml O2k-chamber: 2 µg/ml

@@ Line 12: / Line 12: @@
 |type=Inhibitor ETS
 }}
+== Application in [[HRR]] ==
+'''Mna: Malonate''' (Malonic acid, CH<sub>2</sub>(COOH)<sub>2</sub>); Sigma M 1296, 5 g, store at -20 °C: FW = 800)
+<span style="color:#8B008B"> '''Caution:''' Very toxic. Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.</span>
+'''Preparation of 4 mg/ml stock solution''' (dissolved in H<sub>2</sub>0):
+::1) weigh 4 mg of Malonic acid directly into a 2 ml glass vial.
+::2) add 1 ml H<sub>2</sub>0
+::3) divide into 0.2 ml portions
+::4) store frozen at -20 °C
+'''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]
+::* Titration volume: 1 µl using a 10 µl syringe
+::* Final conc. in 2 ml O2k-chamber: 2 µg/ml