Difference between revisions of "Malonate"

Revision as of 15:49, 2 May 2014

high-resolution terminology - matching measurements at high-resolution

Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).

Abbreviation: Mna

Reference: MiPNet09.12

MitoPedia topics: Inhibitor

Application in HRR

Mna: Malonic acid ( CH₂(COOH)₂); Sigma M 1296, 5 g, store at R.T.; FW = 104.06.

Recommendation: Prepare fresh everyday!

Preparation of 2 M stock solution (dissolved in H20):

1) Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µl).

2) Dissolve in 75 µl 5 N KOH and check pH.

3) Titrate small volumes (2 µl) of 5 N KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).

4) Add H₂O to a final volume of 100 µl.

Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
Final concentration: 5 mM.

@@ Line 4: / Line 4: @@
 |info=[[MiPNet09.12]]
 |type=Inhibitor ETS
+}}
+{{MitoPedia methods|type=Inhibitor ETS
 }}
 {{MitoPedia topics
@@ Line 9: / Line 11: @@
 |type=Inhibitor ETS
 }}
 == Application in [[HRR]] ==
-'''Mna: Malonic acid''' ( CH<sub>2</sub>(COOH)<sub>2</sub>); Sigma M 1296, 5 g, store at R.T.; FW = 104.06
+'''Mna: Malonic acid''' ( CH<sub>2</sub>(COOH)<sub>2</sub>); Sigma M 1296, 5 g, store at R.T.; FW = 104.06.
@@ Line 20: / Line 21: @@
 '''Preparation of 2 M stock solution''' (dissolved in H20):
-::1) weigh 20.8 mg of Malonic acid directly into a Eppendorf tube of appropriate volume (final volume = 100 µl)
+::1) Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µl).
-::2) dissolve in 75 µl 5 N KOH and check pH
+::2) Dissolve in 75 µl 5 N KOH and check pH.
-::3) titrate small amounts (2 µl) of 5 N KOH until you reach a pH of 6.0  (A pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very little amounts of excess KOH.)
+::3) Titrate small volumes (2 µl) of 5 N KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
-::4) add H<sub>2</sub>O to a final volume of 100 µl
+::4) Add H<sub>2</sub>O to a final volume of 100 µl.
-'''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]
+'''Oxygraph-2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::* Titration volume: 5 µl using a 25 µl syringe
+::* Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
-::* Final conc. in 2 ml O2k-chamber: 5 mM
+::* Final concentration: 5 mM.