Difference between revisions of "Malonate"

Revision as of 17:30, 18 February 2014

high-resolution terminology - matching measurements at high-resolution

Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).

Abbreviation: Mna

Reference: MiPNet09.12

MitoPedia topics: Inhibitor

Application in HRR

Mna: Malonic acid ( CH₂(COOH)₂); Sigma M 1296, 5 g, store at R.T.; FW = 104.06

Recommendation: Prepare fresh everyday!

Preparation of 2 M stock solution (dissolved in H20):

1) weigh 20.8 mg of Malonic acid directly into a Eppendorf tube of appropriate volume (final volume = 100 µl)

2) dissolve in 75 µl 5 N KOH and check pH

3) titrate small amounts (2 µl) of 5 N KOH until you reach a pH of 6.0 (A pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very little amounts of excess KOH.)

4) add H₂O to a final volume of 100 µl

Oxygraph-2k Manual Titrations: MiPNet09.12

Titration volume: 5 µl using a 25 µl syringe
Final conc. in 2 ml O2k-chamber: 5 mM

@@ Line 21: / Line 21: @@
 ::1) weigh 20.8 mg of Malonic acid directly into a Eppendorf tube of appropriate volume (final volume = 100 µl)
-::2) dissolve in 20 µl H<sub>2</sub>O
+::2) dissolve in 75 µl 5 N KOH and check pH
-::3) add 70 µl 5 N KOH and check pH
+::3) titrate small amounts (2 µl) of 5 N KOH until you reach a pH of 6.0  (A pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very little amounts of excess KOH.)
-::4) titrate small amounts (2 µl) of 5 N KOH until you reach a pH of 6.0 (approx. 4 titration steps). A pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very little amounts of excess KOH.
+::4) add H<sub>2</sub>O to a final volume of 100 µl
-::5) add H<sub>2</sub>O to a final volume of 100 µl