Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Malonate"

From Bioblast
Line 9: Line 9:
|type=Inhibitor ETS
|type=Inhibitor ETS
}}
}}
== Application in [[HRR]] ==
== Application in [[HRR]] ==


'''Mna: Malonate''' (Malonic acid, CH<sub>2</sub>(COOH)<sub>2</sub>); Sigma M 1296, 5 g, store at R.T.: FW = 800
'''Mna: Malonate''' (Malonic acid, CH<sub>2</sub>(COOH)<sub>2</sub>); Sigma M 1296, 5 g, store at R.T.; FW = 104.06


<span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>
<span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>

Revision as of 08:09, 18 May 2012


high-resolution terminology - matching measurements at high-resolution


Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).

Abbreviation: Mna

Reference: MiPNet09.12


MitoPedia topics: Inhibitor 


Application in HRR

Mna: Malonate (Malonic acid, CH2(COOH)2); Sigma M 1296, 5 g, store at R.T.; FW = 104.06

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


Preparation of 2 M stock solution (dissolved in H20): Recommended to prepare fresh every day

1) weigh 20.8 mg of Malonic acid directly into a Eppendorf tube of appropriate volume (final volume = 100 µl)
2) dissolve in 20 µl H2O
3) add 70 µl 5 N KOH and check pH
4) titrate small amounts (2 µl) of 5 N KOH until you reach a pH of 6.0 (approx. 4 titration steps). A pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very little amounts of excess KOH.
5) add H2O to a final volume of 100 µl


Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 5 µl using a 10 µl syringe
  • Final conc. in 2 ml O2k-chamber: 5 mM