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Difference between revisions of "Malonate"

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== Application in [[HRR]] ==
== Application in [[HRFR]] ==


=== Preparation and titration ===
=== Preparation and titration ===

Revision as of 13:31, 29 August 2017


high-resolution terminology - matching measurements at high-resolution


Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of succinate dehydrogenase (Complex II). Malonate is a substrate of malonyl-CoA synthase.

Abbreviation: Mna

Reference: MiPNet09.12


MitoPedia topics: Inhibitor 

Application in HRFR

Preparation and titration

Mna: Malonic acid ( CH2(COOH)2); Sigma M 1296, 5 g, store at R.T.; FW = 104.06.

Recommendation: Prepare fresh everyday!

Preparation of 2 M stock solution (dissolved in H20):
  1. Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µl).
  2. Dissolve in 85 µl 5 M KOH and check pH.
  3. Titrate small volumes (2 µl) of 5 M KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
  4. Add H2O to a final volume of 100 µl.
O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
  • Final concentration: 5 mM.

Malonic acid and malonyl-CoA synthase

Communicated by Garcia e Souza LF 2016-11-11
Activity of mt-malonyl-CoA synthase (Witkowski et al 2011) in a mt-preparation may cause stimulation of respiration after titration of malonate (unpublished observation).
Reference
  • Witkowski A, Thweatt J, Smith S (2011) Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis. J Biol Chem 286:33729-36. - »Bioblast link«