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Difference between revisions of "Malonate"

From Bioblast
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{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Inhibitor
|mitopedia topic=Inhibitor
|type=Inhibitor ETS
}}
{{Labeling
|discipline=Mitochondrial Physiology
|enzymes=Complex II; Succinate Dehydrogenase
|kinetics=Inhibitor; Uncoupler
|instruments=Chemicals; Media
|type=Inhibitor ETS
|type=Inhibitor ETS
}}
}}

Revision as of 15:51, 9 March 2011


high-resolution terminology - matching measurements at high-resolution


Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).

Abbreviation: Mna

Reference: MiPNet09.12


MitoPedia topics: Inhibitor 

Application in HRR

Mna: Malonate (Malonic acid, CH2(COOH)2); Sigma M 1296, 5 g, store at R.T.: FW = 800

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


Preparation of 2 M stock solution (dissolved in H20): Recommended to prepare fresh every day

1) weigh 20.8 mg of Malonic acid directly into a Eppendorf tube of appropriate volume (final volume = 100 µl)
2) dissolve in 20 µl H2O
3) add 70 µl 5 N KOH and check pH
4) titrate small amounts (2 µl) of 5 N KOH until you reach a pH of 6.0 (approx. 4 titration steps). A pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very little amounts of excess KOH.
5) add H2O to a final volume of 100 µl


Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 5 µl using a 10 µl syringe
  • Final conc. in 2 ml O2k-chamber: 5 mM