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Difference between revisions of "Malonate"

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::1) Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µl).
::1) Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µl).
::2) Dissolve in 85 µl 5 N KOH and check pH.
::2) Dissolve in 85 µl 5 M KOH and check pH.
::3) Titrate small volumes (2 µl) of 5 N KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
::3) Titrate small volumes (2 µl) of 5 M KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
::4) Add H<sub>2</sub>O to a final volume of 100 µl.
::4) Add H<sub>2</sub>O to a final volume of 100 µl.



Revision as of 11:01, 26 August 2016


high-resolution terminology - matching measurements at high-resolution


Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).

Abbreviation: Mna

Reference: MiPNet09.12



MitoPedia topics: Inhibitor 

Application in HRR

Mna: Malonic acid ( CH2(COOH)2); Sigma M 1296, 5 g, store at R.T.; FW = 104.06.


Recommendation: Prepare fresh everyday!


Preparation of 2 M stock solution (dissolved in H20):

1) Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µl).
2) Dissolve in 85 µl 5 M KOH and check pH.
3) Titrate small volumes (2 µl) of 5 M KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
4) Add H2O to a final volume of 100 µl.


O2k manual titrations MiPNet09.12 O2k-Titrations

  • Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
  • Final concentration: 5 mM.