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Difference between revisions of "Malonate"

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Revision as of 13:59, 9 March 2011


high-resolution terminology - matching measurements at high-resolution


Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).

Abbreviation: Mna

Reference: MiPNet09.12


MitoPedia topics: Inhibitor 


Labels:



Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. 


HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 


Application in HRR

Mna: Malonate (Malonic acid, CH2(COOH)2); Sigma M 1296, 5 g, store at R.T.: FW = 800

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


Preparation of 2 M stock solution (dissolved in H20): Recommended to prepare fresh every day

1) weigh 20.8 mg of Malonic acid directly into a Eppendorf tube of appropriate volume (final volume = 100 µl)
2) dissolve in 20 µl H2O
3) add 70 µl 5 N KOH and check pH
4) titrate small amounts (2 µl) of 5 N KOH until you reach a pH of 6.0 (approx. 4 titration steps). A pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very little amounts of excess KOH.
5) add H2O to a final volume of 100 µl


Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 5 µl using a 10 µl syringe
  • Final conc. in 2 ml O2k-chamber: 5 mM