Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Larsen 2014 Am J Clin Nutr"

From Bioblast
Line 5: Line 5:
|year=2014
|year=2014
|journal=Am J Clin Nutr
|journal=Am J Clin Nutr
|abstract=BACKGROUND:
|abstract=Nitrate, which is an inorganic anion abundant in vegetables, increases the efficiency of isolated human mitochondria. Such an effect might be reflected in changes in the resting metabolic rate (RMR) and formation of reactive oxygen species. The bioactivation of nitrate involves its active accumulation in saliva followed by a sequential reduction to nitrite, nitric oxide, and other reactive nitrogen species.
Nitrate, which is an inorganic anion abundant in vegetables, increases the efficiency of isolated human mitochondria. Such an effect might be reflected in changes in the resting metabolic rate (RMR) and formation of reactive oxygen species. The bioactivation of nitrate involves its active accumulation in saliva followed by a sequential reduction to nitrite, nitric oxide, and other reactive nitrogen species.


OBJECTIVE:
We studied effects of inorganic nitrate, in amounts that represented a diet rich in vegetables, on the RMR in healthy volunteers.
We studied effects of inorganic nitrate, in amounts that represented a diet rich in vegetables, on the RMR in healthy volunteers.


DESIGN:
In a randomized, double-blind, crossover study, we measured the RMR by using indirect calorimetry in 13 healthy volunteers after a 3-d dietary intervention with sodium nitrate (NaNO3) or a placebo (NaCl). The nitrate dose (0.1 mmol β‹… kg-1 β‹… d-1) corresponded to the amount in 200-300 g spinach, beetroot, lettuce, or other vegetable that was rich in nitrate. Effects of direct nitrite exposure on cell respiration were studied in cultured human primary myotubes.
In a randomized, double-blind, crossover study, we measured the RMR by using indirect calorimetry in 13 healthy volunteers after a 3-d dietary intervention with sodium nitrate (NaNO3) or a placebo (NaCl). The nitrate dose (0.1 mmol β‹… kg-1 β‹… d-1) corresponded to the amount in 200-300 g spinach, beetroot, lettuce, or other vegetable that was rich in nitrate. Effects of direct nitrite exposure on cell respiration were studied in cultured human primary myotubes.


RESULTS:
The RMR was 4.2% lower after nitrate compared with placebo administration, and the change correlated strongly to the degree of nitrate accumulation in saliva (r2 = 0.71). The thyroid hormone status, insulin sensitivity, glucose uptake, plasma concentration of isoprostanes, and total antioxidant capacity were unaffected by nitrate. The administration of nitrite to human primary myotubes acutely inhibited respiration.
The RMR was 4.2% lower after nitrate compared with placebo administration, and the change correlated strongly to the degree of nitrate accumulation in saliva (r2 = 0.71). The thyroid hormone status, insulin sensitivity, glucose uptake, plasma concentration of isoprostanes, and total antioxidant capacity were unaffected by nitrate. The administration of nitrite to human primary myotubes acutely inhibited respiration.


CONCLUSIONS:
Dietary inorganic nitrate reduces the RMR. This effect may have implications for the regulation of metabolic function in health and disease.
Dietary inorganic nitrate reduces the RMR. This effect may have implications for the regulation of metabolic function in health and disease.
|mipnetlab=SE Stockholm Sahlin K, SE Stockholm Weitzberg E
|mipnetlab=SE Stockholm Sahlin K, SE Stockholm Weitzberg E
Line 24: Line 19:
|area=Respiration
|area=Respiration
|organism=Human
|organism=Human
|tissues=Heart
|tissues=Skeletal muscle
|model cell lines=Stem cells
|model cell lines=Stem cells
|preparations=Intact cells, Permeabilized cells
|preparations=Intact cells, Permeabilized cells

Revision as of 13:39, 5 August 2015

Publications in the MiPMap
Larsen FJ, Schiffer TA, Ekblom B, Mattsson MP, Checa A, Wheelock CE, NystrΓΆm T, Lundberg JO, Weitzberg E (2014) Dietary nitrate reduces resting metabolic rate: a randomized, crossover study in humans. Am J Clin Nutr 99:843-50.

Β» PMID: 24500154

Larsen FJ, Schiffer TA, Ekblom B, Mattsson MP, Checa A, Wheelock CE, Nystroem T, Lundberg JO, Weitzberg E (2014) Am J Clin Nutr

Abstract: Nitrate, which is an inorganic anion abundant in vegetables, increases the efficiency of isolated human mitochondria. Such an effect might be reflected in changes in the resting metabolic rate (RMR) and formation of reactive oxygen species. The bioactivation of nitrate involves its active accumulation in saliva followed by a sequential reduction to nitrite, nitric oxide, and other reactive nitrogen species.

We studied effects of inorganic nitrate, in amounts that represented a diet rich in vegetables, on the RMR in healthy volunteers.

In a randomized, double-blind, crossover study, we measured the RMR by using indirect calorimetry in 13 healthy volunteers after a 3-d dietary intervention with sodium nitrate (NaNO3) or a placebo (NaCl). The nitrate dose (0.1 mmol β‹… kg-1 β‹… d-1) corresponded to the amount in 200-300 g spinach, beetroot, lettuce, or other vegetable that was rich in nitrate. Effects of direct nitrite exposure on cell respiration were studied in cultured human primary myotubes.

The RMR was 4.2% lower after nitrate compared with placebo administration, and the change correlated strongly to the degree of nitrate accumulation in saliva (r2 = 0.71). The thyroid hormone status, insulin sensitivity, glucose uptake, plasma concentration of isoprostanes, and total antioxidant capacity were unaffected by nitrate. The administration of nitrite to human primary myotubes acutely inhibited respiration.

Dietary inorganic nitrate reduces the RMR. This effect may have implications for the regulation of metabolic function in health and disease.


β€’ O2k-Network Lab: SE Stockholm Sahlin K, SE Stockholm Weitzberg E


Labels: MiParea: Respiration 


Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Intact cells, Permeabilized cells 


Coupling state: LEAK, ROUTINE, OXPHOS 

HRR: Oxygraph-2k