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Difference between revisions of "Lanza 2011 J Magnetic Resonance Imaging"

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{{Publication
{{Publication
|title=Lanza IR, Bhagra S, Nair KS, Port JD (2011) Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy: A cross-validation with in vitro measurements.. J. Magnetic Resonance Imaging DOI: 10.1002/jmri.22733.
|title=Lanza IR, Bhagra S, Nair KS, Port JD (2011) Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy: A cross-validation with in vitro measurements. J Magnetic Resonance Imaging DOI: 10.1002/jmri.22733.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/19426878 PMID:19426878 ]
|info=[http://www.ncbi.nlm.nih.gov/pubmed/22006551 PMID:22006551 ]
|authors=Lanza IR, Bhagra S, Nair KS, Port JD
|authors=Lanza IR, Bhagra S, Nair KS, Port JD
|year=2011
|year=2011
|journal=J. Magnetic Resonance Imaging
|journal=J Magnetic Resonance Imaging
|abstract=Purpose: To cross-validate skeletal muscle oxidative capacity measured by 31P-MR spectroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults.
|abstract=Purpose: To cross-validate skeletal muscle oxidative capacity measured by 31P-MR spectroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults.


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}}
}}
{{Labeling
{{Labeling
|organism=Human
|tissues=Skeletal muscle
|preparations=Isolated mitochondria
|couplingstates=OXPHOS
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|organism=Human
|tissues=Skeletal Muscle
|preparations=Permeabilized Cell or Tissue; Homogenate
|topics=Respiration; OXPHOS; ETS Capacity
}}
}}

Latest revision as of 12:12, 24 February 2015

Publications in the MiPMap
Lanza IR, Bhagra S, Nair KS, Port JD (2011) Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy: A cross-validation with in vitro measurements. J Magnetic Resonance Imaging DOI: 10.1002/jmri.22733.

Β» PMID:22006551

Lanza IR, Bhagra S, Nair KS, Port JD (2011) J Magnetic Resonance Imaging

Abstract: Purpose: To cross-validate skeletal muscle oxidative capacity measured by 31P-MR spectroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults.

Materials and Methods: Oxidative capacity in vivo was determined from PCr recovery kinetics following a 30-s maximal isometric knee extension. State 3 respiration was measured in isolated mitochondria using high-resolution respirometry. A second cohort of 10 individuals underwent two 31P-MRS testing sessions to assess the test–retest reproducibility of the method.

Results: Overall, the in vivo and in vitro methods were well-correlated (r = 0.66–0.72) and showed good agreement by Bland Altman plots. Excellent reproducibility was observed for the PCr recovery rate constant (CV = 4.6%; ICC = 0.85) and calculated oxidative capacity (CV = 3.4%; ICC = 0.83).

Conclusion: These results indicate that 31P-MRS corresponds well with gold-standard in vitro measurements and is highly reproducible. β€’ Keywords: phosphocreatine recovery;skeletal muscle;high-resolution respirometry;mitochondria;oxidative capacity

β€’ O2k-Network Lab: US MN Rochester Nair KS


Labels:


Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Isolated mitochondria 


Coupling state: OXPHOS 

HRR: Oxygraph-2k