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Difference between revisions of "Lanza 2011 J Magnetic Resonance Imaging"

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|tissues=Skeletal muscle
|tissues=Skeletal muscle
|preparations=Isolated Mitochondria
|preparations=Isolated Mitochondria
|topics=Respiration; OXPHOS; ETS Capacity
|couplingstates=OXPHOS
}}
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Revision as of 14:24, 27 November 2012

Publications in the MiPMap
Lanza IR, Bhagra S, Nair KS, Port JD (2011) Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy: A cross-validation with in vitro measurements. J Magnetic Resonance Imaging DOI: 10.1002/jmri.22733.

ยป PMID:22006551

Lanza IR, Bhagra S, Nair KS, Port JD (2011) J Magnetic Resonance Imaging

Abstract: Purpose: To cross-validate skeletal muscle oxidative capacity measured by 31P-MR spectroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults.

Materials and Methods: Oxidative capacity in vivo was determined from PCr recovery kinetics following a 30-s maximal isometric knee extension. State 3 respiration was measured in isolated mitochondria using high-resolution respirometry. A second cohort of 10 individuals underwent two 31P-MRS testing sessions to assess the testโ€“retest reproducibility of the method.

Results: Overall, the in vivo and in vitro methods were well-correlated (r = 0.66โ€“0.72) and showed good agreement by Bland Altman plots. Excellent reproducibility was observed for the PCr recovery rate constant (CV = 4.6%; ICC = 0.85) and calculated oxidative capacity (CV = 3.4%; ICC = 0.83).

Conclusion: These results indicate that 31P-MRS corresponds well with gold-standard in vitro measurements and is highly reproducible. โ€ข Keywords: phosphocreatine recovery;skeletal muscle;high-resolution respirometry;mitochondria;oxidative capacity

โ€ข O2k-Network Lab: US MN Rochester Nair KS


Labels:


Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 


Coupling state: OXPHOS 

HRR: Oxygraph-2k