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Difference between revisions of "Lanza 2011 J Magnetic Resonance Imaging"

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Lanza IR, Bhagra S, Nair KS, Port JD (2011) Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy: A cross-validation with in vitro measurements.. J. Magnetic Resonance Imaging DOI: 10.1002/jmri.22733.

ยป PMID:19426878

Lanza IR, Bhagra S, Nair KS, Port JD (2011) J. Magnetic Resonance Imaging

Abstract: Purpose: To cross-validate skeletal muscle oxidative capacity measured by 31P-MR spectroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults.

Materials and Methods: Oxidative capacity in vivo was determined from PCr recovery kinetics following a 30-s maximal isometric knee extension. State 3 respiration was measured in isolated mitochondria using high-resolution respirometry. A second cohort of 10 individuals underwent two 31P-MRS testing sessions to assess the testโ€“retest reproducibility of the method.

Results: Overall, the in vivo and in vitro methods were well-correlated (r = 0.66โ€“0.72) and showed good agreement by Bland Altman plots. Excellent reproducibility was observed for the PCr recovery rate constant (CV = 4.6%; ICC = 0.85) and calculated oxidative capacity (CV = 3.4%; ICC = 0.83).

Conclusion: These results indicate that 31P-MRS corresponds well with gold-standard in vitro measurements and is highly reproducible. โ€ข Keywords: phosphocreatine recovery;skeletal muscle;high-resolution respirometry;mitochondria;oxidative capacity

โ€ข O2k-Network Lab: US MN Rochester Nair KS


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Organism: Human  Tissue;cell: Skeletal Muscle"Skeletal Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 

Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Oxygraph-2k