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Difference between revisions of "Lanza 2010 Curr Opin Clin Nutr Metab Care"

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{{Publication
{{Publication
|title=Lanza IR, Nair KS. (2010) Mitochondrial metabolic function assessed in vivo and in vitro. Curr. Opin. Clin. Nutr Metab.Care.13(5):511-517.
|title=Lanza IR, Nair KS (2010) Mitochondrial metabolic function assessed ''in vivo'' and ''in vitro''. Curr Opin Clin Nutr Metab Care 13:511-7.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/20616711 PMID:20616711]
|info=[http://www.ncbi.nlm.nih.gov/pubmed/20616711 PMID: 20616711 Open Access]
|authors=Lanza IR, Nair KS
|authors=Lanza IR, Nair KS
|year=2010
|year=2010
|journal=Opin. Clin. Nutr Metab. Care
|journal=Curr Opin Clin Nutr Metab Care
|abstract=Purpose of review: Mitochondrial content and function vary across species, tissue types, and lifespan. Alterations in skeletal muscle mitochondrial function have been reported to occur in in aging and in many other pathological conditions. This review focuses on the state of the art in vivo and in vitro methodologies for assessment of muscle mitochondrial function.
|abstract='''Purpose of review''': Mitochondrial content and function vary across species, tissue types, and lifespan. Alterations in skeletal muscle mitochondrial function have been reported to occur in in aging and in many other pathological conditions. This review focuses on the state of the art ''in vivo'' and ''in vitro'' methodologies for assessment of muscle mitochondrial function.


Recent findings: Classic studies of isolated mitochondria have measured function from maximal respiratory capacity. These fundamental methods have recently been substantially improved and novel approaches to asses mitochondrial functions in vitro have been emerged. Noninvasive
'''Recent findings''': Classic studies of isolated mitochondria have measured function from maximal respiratory capacity. These fundamental methods have recently been substantially improved and novel approaches to asses mitochondrial functions ''in vitro'' have been emerged. Noninvasive
methods based on magnetic resonance spectroscopy (MRS) and near-infrared
methods based on magnetic resonance spectroscopy (MRS) and near-infrared
spectroscopy (NIRS) permit in vivo assessment of mitochondrial function and are rapidly becoming more accessible to many investigators. Moreover, it is now possible to gather information on regulation of mitochondrial content by measuring the in vivo synthesis rate of individual mitochondrial proteins.
spectroscopy (NIRS) permit ''in vivo'' assessment of mitochondrial function and are rapidly becoming more accessible to many investigators. Moreover, it is now possible to gather information on regulation of mitochondrial content by measuring the ''in vivo'' synthesis rate of individual mitochondrial proteins.


Summary: High-resolution respirometry has emerged as a powerful tool for in vitro measurements of mitochondrial function in isolated mitochondria and permeabilized fibers. Direct measurements of ATP production are possible by bioluminescence. Mechanistic data provided by these methods is further complimented by in vivo assessment using MRS and NIRS and the translational rate of gene transcripts.
'''Summary: High-resolution respirometry has emerged as a powerful tool for ''in vitro'' measurements of mitochondrial function in isolated mitochondria and permeabilized fibers.''' Direct measurements of ATP production are possible by bioluminescence. Mechanistic data provided by these methods is further complimented by ''in vivo'' assessment using MRS and NIRS and the translational rate of gene transcripts.
|keywords=mitochondria, skeletal muscle, respiration, magnetic resonance spectroscopy, mass spectrometry
|keywords=Mitochondria, Skeletal muscle, Respiration, Magnetic resonance spectroscopy, Mass spectrometry
|mipnetlab=US_MN Rochester_Nair K
|mipnetlab=US MN Rochester Nair KS
}}
}}
{{Labeling
{{Labeling
|organism=Human
|tissues=Skeletal muscle
|preparations=Permeabilized tissue, Isolated mitochondria
|diseases=Aging;senescence
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|injuries=Aging; Senescence
|organism=Human
|tissues=Skeletal Muscle
|preparations=Isolated Mitochondria, Permeabilized Cell or Tissue; Homogenate
|enzymes=Complex II; Succinate Dehydrogenase
|topics=ATP; ADP; AMP; PCr
}}
}}

Latest revision as of 14:44, 19 March 2015

Publications in the MiPMap
Lanza IR, Nair KS (2010) Mitochondrial metabolic function assessed in vivo and in vitro. Curr Opin Clin Nutr Metab Care 13:511-7.

Β» PMID: 20616711 Open Access

Lanza IR, Nair KS (2010) Curr Opin Clin Nutr Metab Care

Abstract: Purpose of review: Mitochondrial content and function vary across species, tissue types, and lifespan. Alterations in skeletal muscle mitochondrial function have been reported to occur in in aging and in many other pathological conditions. This review focuses on the state of the art in vivo and in vitro methodologies for assessment of muscle mitochondrial function.

Recent findings: Classic studies of isolated mitochondria have measured function from maximal respiratory capacity. These fundamental methods have recently been substantially improved and novel approaches to asses mitochondrial functions in vitro have been emerged. Noninvasive methods based on magnetic resonance spectroscopy (MRS) and near-infrared spectroscopy (NIRS) permit in vivo assessment of mitochondrial function and are rapidly becoming more accessible to many investigators. Moreover, it is now possible to gather information on regulation of mitochondrial content by measuring the in vivo synthesis rate of individual mitochondrial proteins.

Summary: High-resolution respirometry has emerged as a powerful tool for in vitro measurements of mitochondrial function in isolated mitochondria and permeabilized fibers. Direct measurements of ATP production are possible by bioluminescence. Mechanistic data provided by these methods is further complimented by in vivo assessment using MRS and NIRS and the translational rate of gene transcripts. β€’ Keywords: Mitochondria, Skeletal muscle, Respiration, Magnetic resonance spectroscopy, Mass spectrometry

β€’ O2k-Network Lab: US MN Rochester Nair KS


Labels: Pathology: Aging;senescence 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue, Isolated mitochondria 



HRR: Oxygraph-2k