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Laner 2014 Abstract MiP2014 - Revision history
2024-03-29T00:41:42Z
Revision history for this page on the wiki
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Gnaiger Erich at 16:30, 10 January 2022
2022-01-10T16:30:29Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 16:30, 10 January 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ET capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ET capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=<del style="font-weight: bold; text-decoration: none;">AT Innsbruck Gnaiger E, </del>AT Innsbruck Oroboros, CA Vancouver Boushel RC, US OK Stillwater Davis MS, US CO Fort Collins Miller BF, US CO Fort Collins Hamilton K, US OK Oklahoma City Miller BF</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros, CA Vancouver Boushel RC, US OK Stillwater Davis MS, US CO Fort Collins Miller BF, US CO Fort Collins Hamilton K, US OK Oklahoma City Miller BF</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">}}</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">{{Labeling</del></div></td><td colspan="2"></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliation ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliation ==</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837-45.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837-45.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.</div></td></tr>
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<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">{{Labeling</ins></div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Laner_2014_Abstract_MiP2014&diff=187255&oldid=prev
Plangger Mario at 07:24, 4 November 2019
2019-11-04T07:24:21Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ET capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ET capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros, CA Vancouver Boushel RC, US OK Stillwater Davis MS, US CO Fort Collins Miller BF, US CO Fort Collins Hamilton K</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros, CA Vancouver Boushel RC, US OK Stillwater Davis MS, US CO Fort Collins Miller BF, US CO Fort Collins Hamilton K<ins style="font-weight: bold; text-decoration: none;">, US OK Oklahoma City Miller BF</ins></div></td></tr>
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Plangger Mario
https://wiki.oroboros.at/index.php?title=Laner_2014_Abstract_MiP2014&diff=179365&oldid=prev
Gnaiger C at 07:26, 24 May 2019
2019-05-24T07:26:32Z
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|title=Cytochrome c flux control factor as a quality criterion in respiratory OXPHOS analysis in canine permeabilized <del style="font-weight: bold; text-decoration: none;">fibres</del>.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|title=Cytochrome c flux control factor as a quality criterion in respiratory OXPHOS analysis in canine permeabilized <ins style="font-weight: bold; text-decoration: none;">fibers</ins>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[File:VG.jpg|150px|right|Laner V]] [[Laner 2014 Mitochondr Physiol Network MiP2014|Mitochondr Physiol Network 19.13]] - [http://www.mitophysiology.org/index.php?mip2014 MiP2014]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[File:VG.jpg|150px|right|Laner V]] [[Laner 2014 Mitochondr Physiol Network MiP2014|Mitochondr Physiol Network 19.13]] - [http://www.mitophysiology.org/index.php?mip2014 MiP2014]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Laner V, Boushel RC, Hamilton KL, Miller BF, Williamson KK, Davis MS, Gnaiger E</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Laner V, Boushel RC, Hamilton KL, Miller BF, Williamson KK, Davis MS, Gnaiger E</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2014</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2014</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=MiP2014</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=MiP2014</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) preparations (isolated mitochondria, permeabilized cells and tissues, tissue homogenates) provide a fundamental basis for comprehensive OXPHOS analysis for the study of substrate and coupling control of mitochondrial respiration [1]. Plasma membrane permeabilization with mechanical separation of muscle <del style="font-weight: bold; text-decoration: none;">fibre </del>bundles and chemical permeabilization with mild detergents may influence the integrity of the outer mt-membrane and thus induce partial release of cytochrome ''c'' (''c''). In mitochondria isolated from healthy skeletal muscle, CI&II-linked OXPHOS capacity decreases linearly with cytochrome ''c'' loss during isolation [2]. The cytochrome ''c'' effect is expressed as the flux control factor ''FCF<sub>c</sub>'', which is the increase of OXPHOS capacity after addition of 10 µM ''c'' normalized for ''c''-stimulated respiration [1-3]. There is no consensus as to the threshold of ''FCF<sub>c</sub>'' applied as a quantitative exclusion criterion in permeabilized <del style="font-weight: bold; text-decoration: none;">fibres </del>obtained from healthy muscle tissue.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) preparations (isolated mitochondria, permeabilized cells and tissues, tissue homogenates) provide a fundamental basis for comprehensive OXPHOS analysis for the study of substrate and coupling control of mitochondrial respiration [1]. Plasma membrane permeabilization with mechanical separation of muscle <ins style="font-weight: bold; text-decoration: none;">fiber </ins>bundles and chemical permeabilization with mild detergents may influence the integrity of the outer mt-membrane and thus induce partial release of cytochrome ''c'' (''c''). In mitochondria isolated from healthy skeletal muscle, CI&II-linked OXPHOS capacity decreases linearly with cytochrome ''c'' loss during isolation [2]. The cytochrome ''c'' effect is expressed as the flux control factor ''FCF<sub>c</sub>'', which is the increase of OXPHOS capacity after addition of 10 µM ''c'' normalized for ''c''-stimulated respiration [1-3]. There is no consensus as to the threshold of ''FCF<sub>c</sub>'' applied as a quantitative exclusion criterion in permeabilized <ins style="font-weight: bold; text-decoration: none;">fibers </ins>obtained from healthy muscle tissue.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>We aimed at establishing a reference method for the application of a cytochrome ''c'' threshold as exclusion criterion in mitochondrial OXPHOS analyses. Our study involved Alaskan sled dogs (''N''=6) studied 72 to 120 h after finishing a competitive 1,000 mile race in nine days. Permeabilized <del style="font-weight: bold; text-decoration: none;">fibres </del>(wet weight per chamber of 0.81-1.28 mg ± 0.12 SD) were prepared from needle biopsies and immediately studied by high-resolution respirometry [4] using 12 chambers in parallel (Oroboros Oxygraph-2k). Compared to human skeletal muscle <del style="font-weight: bold; text-decoration: none;">fibres</del>, the canine samples were more trexturally supple and sticky, requiring delicate fiber separation under light microscope, and disintegrating to various degrees during substrate-uncoupler-inhibitor titration (SUIT) protocols. This was reflected in variable and sometimes extremely high cytochrome ''c'' effects. However, there was no loss of CI- or CI&II-linked OXPHOS and ET capacity with increasing ''FCF<sub>c</sub>'' (Figure 1). Apparently, the damage caused by mt-preparation even in cases with ''FCF<sub>c</sub>'' up to 0.25 could be rescued by addition of 10 µM ''c'' and thus restore capacities comparable with samples of negligible ''FCF<sub>c</sub>''. In contrast, multiple defects associated with increasing ''FCF<sub>c</sub>'' in human muscle <del style="font-weight: bold; text-decoration: none;">fibres </del>cannot be compensated fully by addition of cytochrome ''c'' [2,5]. Cytochrome ''c'' was applied early in the two SUIT protocols, in the CI-linked or CI&FAO-linked OXPHOS state. This allowed consistent analysis of subsequent respiratory states which were all supported by the externally added cytochrome ''c'' (Figure 1).</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>We aimed at establishing a reference method for the application of a cytochrome ''c'' threshold as exclusion criterion in mitochondrial OXPHOS analyses. Our study involved Alaskan sled dogs (''N''=6) studied 72 to 120 h after finishing a competitive 1,000 mile race in nine days. Permeabilized <ins style="font-weight: bold; text-decoration: none;">fibers </ins>(wet weight per chamber of 0.81-1.28 mg ± 0.12 SD) were prepared from needle biopsies and immediately studied by high-resolution respirometry [4] using 12 chambers in parallel (Oroboros Oxygraph-2k). Compared to human skeletal muscle <ins style="font-weight: bold; text-decoration: none;">fibers</ins>, the canine samples were more trexturally supple and sticky, requiring delicate fiber separation under light microscope, and disintegrating to various degrees during substrate-uncoupler-inhibitor titration (SUIT) protocols. This was reflected in variable and sometimes extremely high cytochrome ''c'' effects. However, there was no loss of CI- or CI&II-linked OXPHOS and ET capacity with increasing ''FCF<sub>c</sub>'' (Figure 1). Apparently, the damage caused by mt-preparation even in cases with ''FCF<sub>c</sub>'' up to 0.25 could be rescued by addition of 10 µM ''c'' and thus restore capacities comparable with samples of negligible ''FCF<sub>c</sub>''. In contrast, multiple defects associated with increasing ''FCF<sub>c</sub>'' in human muscle <ins style="font-weight: bold; text-decoration: none;">fibers </ins>cannot be compensated fully by addition of cytochrome ''c'' [2,5]. Cytochrome ''c'' was applied early in the two SUIT protocols, in the CI-linked or CI&FAO-linked OXPHOS state. This allowed consistent analysis of subsequent respiratory states which were all supported by the externally added cytochrome ''c'' (Figure 1).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ET capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ET capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with reference to tissue function. Mol Cell Biochem 174: 55-60.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with reference to tissue function. Mol Cell Biochem 174: 55-60.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–41.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–41.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div># Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized <del style="font-weight: bold; text-decoration: none;">fibres </del>from small biopsies of human muscle. Methods Mol Biol 810: 25-58.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div># Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized <ins style="font-weight: bold; text-decoration: none;">fibers </ins>from small biopsies of human muscle. Methods Mol Biol 810: 25-58.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837-45.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837-45.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.</div></td></tr>
</table>
Gnaiger C
https://wiki.oroboros.at/index.php?title=Laner_2014_Abstract_MiP2014&diff=171141&oldid=prev
Beno Marija at 11:06, 23 January 2019
2019-01-23T11:06:39Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:06, 23 January 2019</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l7">Line 7:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) preparations (isolated mitochondria, permeabilized cells and tissues, tissue homogenates) provide a fundamental basis for comprehensive OXPHOS analysis for the study of substrate and coupling control of mitochondrial respiration [1]. Plasma membrane permeabilization with mechanical separation of muscle fibre bundles and chemical permeabilization with mild detergents may influence the integrity of the outer mt-membrane and thus induce partial release of cytochrome ''c'' (''c''). In mitochondria isolated from healthy skeletal muscle, CI&II-linked OXPHOS capacity decreases linearly with cytochrome ''c'' loss during isolation [2]. The cytochrome ''c'' effect is expressed as the flux control factor ''FCF<sub>c</sub>'', which is the increase of OXPHOS capacity after addition of 10 µM ''c'' normalized for ''c''-stimulated respiration [1-3]. There is no consensus as to the threshold of ''FCF<sub>c</sub>'' applied as a quantitative exclusion criterion in permeabilized fibres obtained from healthy muscle tissue.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) preparations (isolated mitochondria, permeabilized cells and tissues, tissue homogenates) provide a fundamental basis for comprehensive OXPHOS analysis for the study of substrate and coupling control of mitochondrial respiration [1]. Plasma membrane permeabilization with mechanical separation of muscle fibre bundles and chemical permeabilization with mild detergents may influence the integrity of the outer mt-membrane and thus induce partial release of cytochrome ''c'' (''c''). In mitochondria isolated from healthy skeletal muscle, CI&II-linked OXPHOS capacity decreases linearly with cytochrome ''c'' loss during isolation [2]. The cytochrome ''c'' effect is expressed as the flux control factor ''FCF<sub>c</sub>'', which is the increase of OXPHOS capacity after addition of 10 µM ''c'' normalized for ''c''-stimulated respiration [1-3]. There is no consensus as to the threshold of ''FCF<sub>c</sub>'' applied as a quantitative exclusion criterion in permeabilized fibres obtained from healthy muscle tissue.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>We aimed at establishing a reference method for the application of a cytochrome ''c'' threshold as exclusion criterion in mitochondrial OXPHOS analyses. Our study involved Alaskan sled dogs (''N''=6) studied 72 to 120 h after finishing a competitive 1,000 mile race in nine days. Permeabilized fibres (wet weight per chamber of 0.81-1.28 mg ± 0.12 SD) were prepared from needle biopsies and immediately studied by high-resolution respirometry [4] using 12 chambers in parallel (<del style="font-weight: bold; text-decoration: none;">OROBOROS </del>Oxygraph-2k). Compared to human skeletal muscle fibres, the canine samples were more trexturally supple and sticky, requiring delicate fiber separation under light microscope, and disintegrating to various degrees during substrate-uncoupler-inhibitor titration (SUIT) protocols. This was reflected in variable and sometimes extremely high cytochrome ''c'' effects. However, there was no loss of CI- or CI&II-linked OXPHOS and ET capacity with increasing ''FCF<sub>c</sub>'' (Figure 1). Apparently, the damage caused by mt-preparation even in cases with ''FCF<sub>c</sub>'' up to 0.25 could be rescued by addition of 10 µM ''c'' and thus restore capacities comparable with samples of negligible ''FCF<sub>c</sub>''. In contrast, multiple defects associated with increasing ''FCF<sub>c</sub>'' in human muscle fibres cannot be compensated fully by addition of cytochrome ''c'' [2,5]. Cytochrome ''c'' was applied early in the two SUIT protocols, in the CI-linked or CI&FAO-linked OXPHOS state. This allowed consistent analysis of subsequent respiratory states which were all supported by the externally added cytochrome ''c'' (Figure 1).</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>We aimed at establishing a reference method for the application of a cytochrome ''c'' threshold as exclusion criterion in mitochondrial OXPHOS analyses. Our study involved Alaskan sled dogs (''N''=6) studied 72 to 120 h after finishing a competitive 1,000 mile race in nine days. Permeabilized fibres (wet weight per chamber of 0.81-1.28 mg ± 0.12 SD) were prepared from needle biopsies and immediately studied by high-resolution respirometry [4] using 12 chambers in parallel (<ins style="font-weight: bold; text-decoration: none;">Oroboros </ins>Oxygraph-2k). Compared to human skeletal muscle fibres, the canine samples were more trexturally supple and sticky, requiring delicate fiber separation under light microscope, and disintegrating to various degrees during substrate-uncoupler-inhibitor titration (SUIT) protocols. This was reflected in variable and sometimes extremely high cytochrome ''c'' effects. However, there was no loss of CI- or CI&II-linked OXPHOS and ET capacity with increasing ''FCF<sub>c</sub>'' (Figure 1). Apparently, the damage caused by mt-preparation even in cases with ''FCF<sub>c</sub>'' up to 0.25 could be rescued by addition of 10 µM ''c'' and thus restore capacities comparable with samples of negligible ''FCF<sub>c</sub>''. In contrast, multiple defects associated with increasing ''FCF<sub>c</sub>'' in human muscle fibres cannot be compensated fully by addition of cytochrome ''c'' [2,5]. Cytochrome ''c'' was applied early in the two SUIT protocols, in the CI-linked or CI&FAO-linked OXPHOS state. This allowed consistent analysis of subsequent respiratory states which were all supported by the externally added cytochrome ''c'' (Figure 1).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ET capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ET capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck <del style="font-weight: bold; text-decoration: none;">OROBOROS</del>, CA Vancouver Boushel RC, US OK Stillwater Davis MS, US CO Fort Collins Miller BF, US CO Fort Collins Hamilton K</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck <ins style="font-weight: bold; text-decoration: none;">Oroboros</ins>, CA Vancouver Boushel RC, US OK Stillwater Davis MS, US CO Fort Collins Miller BF, US CO Fort Collins Hamilton K</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliation ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliation ==</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>1-<del style="font-weight: bold; text-decoration: none;">OROBOROS INSTRUMENTS</del>, Innsbruck, Austria; 2-The Swedish School Sports Health Sc, Lindigovagen, Sweden; 3-College Health Human Sc, Colorado State Univ., Fort Collins, CO, US; 4-Land O’Lakes Purina Feed, St Louis, MO, US, 5Comparative Exercise Physiol Lab, Center Veterinary Health Sc, Oklahoma State Univ, Stillwater, OK, US; 5D Swarovski Research Lab, Dep Visceral Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria – verena.laner@oroboros.at</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>1-<ins style="font-weight: bold; text-decoration: none;">Oroboros Instruments</ins>, Innsbruck, Austria; 2-The Swedish School Sports Health Sc, Lindigovagen, Sweden; 3-College Health Human Sc, Colorado State Univ., Fort Collins, CO, US; 4-Land O’Lakes Purina Feed, St Louis, MO, US, 5Comparative Exercise Physiol Lab, Center Veterinary Health Sc, Oklahoma State Univ, Stillwater, OK, US; 5D Swarovski Research Lab, Dep Visceral Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria – verena.laner@oroboros.at</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Figures ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Figures ==</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== References and acknowledgements ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== References and acknowledgements ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div> Supported by K-Regio project MitoCom.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div> Supported by K-Regio project MitoCom.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div># Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. <del style="font-weight: bold; text-decoration: none;">OROBOROS </del>MiPNet Publications, Innsbruck: 72 pp. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div># Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. <ins style="font-weight: bold; text-decoration: none;">Oroboros </ins>MiPNet Publications, Innsbruck: 72 pp. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with reference to tissue function. Mol Cell Biochem 174: 55-60.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with reference to tissue function. Mol Cell Biochem 174: 55-60.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–41.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–41.</div></td></tr>
</table>
Beno Marija
https://wiki.oroboros.at/index.php?title=Laner_2014_Abstract_MiP2014&diff=165021&oldid=prev
Beno Marija at 08:20, 30 October 2018
2018-10-30T08:20:38Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ET capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ET capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS, CA Vancouver Boushel RC, US OK Stillwater Davis MS, US CO Fort Collins Miller BF</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS, CA Vancouver Boushel RC, US OK Stillwater Davis MS, US CO Fort Collins Miller BF<ins style="font-weight: bold; text-decoration: none;">, US CO Fort Collins Hamilton K</ins></div></td></tr>
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Beno Marija
https://wiki.oroboros.at/index.php?title=Laner_2014_Abstract_MiP2014&diff=145581&oldid=prev
Kandolf Georg at 13:05, 13 November 2017
2017-11-13T13:05:36Z
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Kandolf Georg
https://wiki.oroboros.at/index.php?title=Laner_2014_Abstract_MiP2014&diff=144232&oldid=prev
Beno Marija at 11:16, 20 October 2017
2017-10-20T11:16:35Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) preparations (isolated mitochondria, permeabilized cells and tissues, tissue homogenates) provide a fundamental basis for comprehensive OXPHOS analysis for the study of substrate and coupling control of mitochondrial respiration [1]. Plasma membrane permeabilization with mechanical separation of muscle fibre bundles and chemical permeabilization with mild detergents may influence the integrity of the outer mt-membrane and thus induce partial release of cytochrome ''c'' (''c''). In mitochondria isolated from healthy skeletal muscle, CI&II-linked OXPHOS capacity decreases linearly with cytochrome ''c'' loss during isolation [2]. The cytochrome ''c'' effect is expressed as the flux control factor ''FCF<sub>c</sub>'', which is the increase of OXPHOS capacity after addition of 10 µM ''c'' normalized for ''c''-stimulated respiration [1-3]. There is no consensus as to the threshold of ''FCF<sub>c</sub>'' applied as a quantitative exclusion criterion in permeabilized fibres obtained from healthy muscle tissue.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondrial (mt) preparations (isolated mitochondria, permeabilized cells and tissues, tissue homogenates) provide a fundamental basis for comprehensive OXPHOS analysis for the study of substrate and coupling control of mitochondrial respiration [1]. Plasma membrane permeabilization with mechanical separation of muscle fibre bundles and chemical permeabilization with mild detergents may influence the integrity of the outer mt-membrane and thus induce partial release of cytochrome ''c'' (''c''). In mitochondria isolated from healthy skeletal muscle, CI&II-linked OXPHOS capacity decreases linearly with cytochrome ''c'' loss during isolation [2]. The cytochrome ''c'' effect is expressed as the flux control factor ''FCF<sub>c</sub>'', which is the increase of OXPHOS capacity after addition of 10 µM ''c'' normalized for ''c''-stimulated respiration [1-3]. There is no consensus as to the threshold of ''FCF<sub>c</sub>'' applied as a quantitative exclusion criterion in permeabilized fibres obtained from healthy muscle tissue.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>We aimed at establishing a reference method for the application of a cytochrome ''c'' threshold as exclusion criterion in mitochondrial OXPHOS analyses. Our study involved Alaskan sled dogs (''N''=6) studied 72 to 120 h after finishing a competitive 1,000 mile race in nine days. Permeabilized fibres (wet weight per chamber of 0.81-1.28 mg ± 0.12 SD) were prepared from needle biopsies and immediately studied by high-resolution respirometry [4] using 12 chambers in parallel (OROBOROS Oxygraph-2k). Compared to human skeletal muscle fibres, the canine samples were more trexturally supple and sticky, requiring delicate fiber separation under light microscope, and disintegrating to various degrees during substrate-uncoupler-inhibitor titration (SUIT) protocols. This was reflected in variable and sometimes extremely high cytochrome ''c'' effects. However, there was no loss of CI- or CI&II-linked OXPHOS and <del style="font-weight: bold; text-decoration: none;">ETS </del>capacity with increasing ''FCF<sub>c</sub>'' (Figure 1). Apparently, the damage caused by mt-preparation even in cases with ''FCF<sub>c</sub>'' up to 0.25 could be rescued by addition of 10 µM ''c'' and thus restore capacities comparable with samples of negligible ''FCF<sub>c</sub>''. In contrast, multiple defects associated with increasing ''FCF<sub>c</sub>'' in human muscle fibres cannot be compensated fully by addition of cytochrome ''c'' [2,5]. Cytochrome ''c'' was applied early in the two SUIT protocols, in the CI-linked or CI&FAO-linked OXPHOS state. This allowed consistent analysis of subsequent respiratory states which were all supported by the externally added cytochrome ''c'' (Figure 1).</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>We aimed at establishing a reference method for the application of a cytochrome ''c'' threshold as exclusion criterion in mitochondrial OXPHOS analyses. Our study involved Alaskan sled dogs (''N''=6) studied 72 to 120 h after finishing a competitive 1,000 mile race in nine days. Permeabilized fibres (wet weight per chamber of 0.81-1.28 mg ± 0.12 SD) were prepared from needle biopsies and immediately studied by high-resolution respirometry [4] using 12 chambers in parallel (OROBOROS Oxygraph-2k). Compared to human skeletal muscle fibres, the canine samples were more trexturally supple and sticky, requiring delicate fiber separation under light microscope, and disintegrating to various degrees during substrate-uncoupler-inhibitor titration (SUIT) protocols. This was reflected in variable and sometimes extremely high cytochrome ''c'' effects. However, there was no loss of CI- or CI&II-linked OXPHOS and <ins style="font-weight: bold; text-decoration: none;">ET </ins>capacity with increasing ''FCF<sub>c</sub>'' (Figure 1). Apparently, the damage caused by mt-preparation even in cases with ''FCF<sub>c</sub>'' up to 0.25 could be rescued by addition of 10 µM ''c'' and thus restore capacities comparable with samples of negligible ''FCF<sub>c</sub>''. In contrast, multiple defects associated with increasing ''FCF<sub>c</sub>'' in human muscle fibres cannot be compensated fully by addition of cytochrome ''c'' [2,5]. Cytochrome ''c'' was applied early in the two SUIT protocols, in the CI-linked or CI&FAO-linked OXPHOS state. This allowed consistent analysis of subsequent respiratory states which were all supported by the externally added cytochrome ''c'' (Figure 1).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and <del style="font-weight: bold; text-decoration: none;">ETS </del>capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and <ins style="font-weight: bold; text-decoration: none;">ET </ins>capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS, CA Vancouver Boushel RC, US OK Stillwater Davis MS, US CO Fort Collins Miller BF</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS, CA Vancouver Boushel RC, US OK Stillwater Davis MS, US CO Fort Collins Miller BF</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Figures ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Figures ==</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[Image:MiP2014_Laner_Figure.jpg|right|400px]] Figure 1. Independence of O2 flux (<del style="font-weight: bold; text-decoration: none;">ETS </del>capacity in the presence of cytochrome ''c'') of the cytochrome ''c'' control factor,</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[Image:MiP2014_Laner_Figure.jpg|right|400px]] Figure 1. Independence of O2 flux (<ins style="font-weight: bold; text-decoration: none;">ET </ins>capacity in the presence of cytochrome ''c'') of the cytochrome ''c'' control factor,</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div> </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''FCF<sub>c</sub>'' = (''J''<sub>CHO''c''</sub>-''J''<sub>CHO</sub>)/''J''<sub>CHO''c''</sub></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''FCF<sub>c</sub>'' = (''J''<sub>CHO''c''</sub>-''J''<sub>CHO</sub>)/''J''<sub>CHO''c''</sub></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">ETS </del>capacity was 238±64 pmol∙s<sup>-1</sup>∙mg<sup>-1</sup> ''W''<sub>w</sub> independent of the CHO substrate combination supporting CI&II-linked electron flow in the presence or absence of 0.2 mM octanoyl carnitine (FAO).</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">ET </ins>capacity was 238±64 pmol∙s<sup>-1</sup>∙mg<sup>-1</sup> ''W''<sub>w</sub> independent of the CHO substrate combination supporting CI&II-linked electron flow in the presence or absence of 0.2 mM octanoyl carnitine (FAO).</div></td></tr>
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Beno Marija
https://wiki.oroboros.at/index.php?title=Laner_2014_Abstract_MiP2014&diff=123863&oldid=prev
Beno Marija at 07:07, 8 November 2016
2016-11-08T07:07:07Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 07:07, 8 November 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|topics=Cyt c</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|topics=Cyt c</div></td></tr>
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Beno Marija
https://wiki.oroboros.at/index.php?title=Laner_2014_Abstract_MiP2014&diff=98643&oldid=prev
Fleischmann Sandra at 06:52, 9 October 2015
2015-10-09T06:52:21Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ETS capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ETS capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS, CA Vancouver Boushel RC, US OK Stillwater Davis MS</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS, CA Vancouver Boushel RC, US OK Stillwater Davis MS<ins style="font-weight: bold; text-decoration: none;">, US CO Fort Collins Miller BF</ins></div></td></tr>
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Fleischmann Sandra
https://wiki.oroboros.at/index.php?title=Laner_2014_Abstract_MiP2014&diff=97131&oldid=prev
Gnaiger Erich at 06:46, 29 August 2015
2015-08-29T06:46:21Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 06:46, 29 August 2015</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ETS capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ETS capacities with FAO- and CI&II-linked substrates were higher than in muscle from competitive horses and humans [5,6]. The present approach (Figure 1) allows evaluation of the ''FCF<sub>c</sub>'' threshold as a potential exclusion criterion in healthy controls.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS<del style="font-weight: bold; text-decoration: none;">, AT Innsbruck MitoCom</del>, CA Vancouver Boushel RC</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS, CA Vancouver Boushel RC<ins style="font-weight: bold; text-decoration: none;">, US OK Stillwater Davis MS</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td></tr>
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Gnaiger Erich