Difference between revisions of "Labajova 2006 Gen Physiol Biophys"
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|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|organism=Rat | |organism=Rat | ||
|tissues= | |tissues=Liver | ||
|preparations=Permeabilized cells | |preparations=Permeabilized cells | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Revision as of 17:34, 25 February 2013
| Lábajová A, Kofránek J, Kriváková P, Cervinková Z, Drahota Z (2006) Tetraphenylphosphonium-Selective Electrode as a Tool for Evaluating Mitochondrial Permeability Transition Pore Function in Isolated Rat Hepatocytes. Gen Physiol Biophys 25: 325-331. |
Labajova A, Kofranek J, Krivakova P, Cervinkova Z, Drahota Z (2006) Gen Physiol Biophys
Abstract: The changes in mitochondrial membrane potential (Δψm) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca2+-induced Δψm dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide. • Keywords: Tetraphenylphosphonium-selective electrode, Mitochondrial permeability transition pore, Hepatocytes
• O2k-Network Lab: CZ_Hradec Kralove_Cervinkova Z
Labels:
Organism: Rat
Tissue;cell: Liver
Preparation: Permeabilized cells
HRR: Oxygraph-2k
