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Difference between revisions of "Labajova 2006 Gen Physiol Biophys"

From Bioblast
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|year=2006
|year=2006
|journal=Gen. Physiol. Biophys.
|journal=Gen. Physiol. Biophys.
|mipnetlab=CZ HradecKralove CervinkovaZ
|abstract=The changes in mitochondrial membrane potential (Δψm) were used as
|abstract=The changes in mitochondrial membrane potential (Δψm) were used as
an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that ''in sit''u mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca<sup>2+</sup>-induced Δψm dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide.
an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that ''in sit''u mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca<sup>2+</sup>-induced Δψm dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide.
|keywords=Tetraphenylphosphonium-selective electrode,  Mitochondrial permeability transition pore,  Hepatocytes
|keywords=Tetraphenylphosphonium-selective electrode,  Mitochondrial permeability transition pore,  Hepatocytes
|mipnetlab=CZ Hradec Kralove Cervinkova Z
|discipline=Mitochondrial Physiology
}}
}}
{{Labeling
{{Labeling
|discipline=Mitochondrial Physiology
|instruments=Oxygraph-2k
|organism=Rat
|organism=Rat
|tissues=Hepatocyte; Liver
|tissues=Hepatocyte; Liver
|topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential
|topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential
|instruments=Oxygraph-2k
|discipline=Mitochondrial Physiology
}}
}}

Revision as of 15:18, 8 August 2011

Publications in the MiPMap
Lábajová A, Kofránek J, Kriváková P, Cervinková Z, Drahota Z (2006) Tetraphenylphosphonium-Selective Electrode as a Tool for Evaluating Mitochondrial Permeability Transition Pore Function in Isolated Rat Hepatocytes. Gen. Physiol. Biophys. 25: 325—331.

» PMID: 17197730

Labajova A, Kofranek J, Krivakova P, Cervinkova Z, Drahota Z (2006) Gen. Physiol. Biophys.

Abstract: The changes in mitochondrial membrane potential (Δψm) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca2+-induced Δψm dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide. Keywords: Tetraphenylphosphonium-selective electrode, Mitochondrial permeability transition pore, Hepatocytes

O2k-Network Lab: CZ Hradec Kralove Cervinkova Z


Labels:


Organism: Rat  Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property. 


Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Oxygraph-2k