Difference between revisions of "Labajova 2006 Anal Biochem"
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|injuries=Cancer; Apoptosis; Cytochrome c | |injuries=Cancer; Apoptosis; Cytochrome c | ||
|organism=Rat | |organism=Rat | ||
|tissues= | |tissues=Liver | ||
|preparations=Permeabilized cells, Isolated Mitochondria | |preparations=Permeabilized cells, Isolated Mitochondria | ||
|additional=Instrumental and methodological aspects | |additional=Instrumental and methodological aspects | ||
|discipline=Biomedicine | |discipline=Biomedicine | ||
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Revision as of 17:34, 25 February 2013
Labajova A, Vojtiskova A, Krivakova P, Kofranek J, Drahota Z, Houstek J (2006) Evaluation of mitochondrial membrane potential using a computerized device with a tetraphenylphosphonium-selective electrode. Anal Biochem 353: 37-42. |
Labajova A, Vojtiskova A, Krivakova P, Kofranek J, Drahota Z, Houstek J (2006) Analyt Biochem
Abstract: Mitochondrial membrane potential (ΞΟm) plays important roles in the normal function of cells and in pathobiochemical situations. The application of ion-selective electrodes for the measurement of ΞΟm is important for studying normal biological reactions and pathways and mitochondrial diseases. We constructed and optimized a computerized device for real-time monitoring of the ΞΟm, which included modification of tetraphenylphosphonium (TPP+)-selective membrane that improved reproducibility of the TPP+-selective electrode. Application of MATLAB software increased the sensitivity of the system. We tested our improved device for membrane potential measurements of isolated mitochondria (in absolute scale of millivolts). In addition, we assessed relative changes of ΞΟm (as changes in TPP+ concentration) of digitonin-permeabilized cells (hepatocytes, control transmitochondrial cybrids, HeLa G and BSC-40) after addition of substrates, inhibitors, and uncoupler of respiratory chain. Our system can be successfully used for studies of many aspects of the regulation of mitochondrial bioenergetics and as a diagnostic tool for mitochondrial oxidative phosphorylation disorders. β’ Keywords: Membrane potential, TPP-selective electrode, Mitochondria, Hepatocytes, Cultured cells
β’ O2k-Network Lab: CZ_Hradec Kralove_Cervinkova Z, CZ_Prague_Houstek J, CZ Prague Bioenergetics
Labels:
Stress:Cancer; Apoptosis; Cytochrome c"Cancer; Apoptosis; Cytochrome c" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Rat Tissue;cell: Liver Preparation: Permeabilized cells, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k, TPP
Instrumental and methodological aspects