Difference between revisions of "Kuznetsov 1998 BTK"
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|tissues=Heart, Skeletal muscle | |tissues=Heart, Skeletal muscle | ||
|preparations=Permeabilized tissue, Isolated mitochondria | |preparations=Permeabilized tissue, Isolated mitochondria | ||
|topics=ADP, O2 | |topics=ADP, O2 | ||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS |
Revision as of 14:06, 16 June 2015
Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, Påhlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:273-6. |
Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Chalmers Reproservice
Abstract: Regulation of oxygen consumption of oxidative muscle is exerted by various factors such as ADP, phosphorylation potential, phosphocreatine/creatine ratio, intracellular Ca2+, nitric oxide and oxygen [1-4]. Permeabilized muscle fibers and isolated cardiomyocytes display pronounced limitation for ADP transport when compared with isolated mitochondria [5]. Whereas the outer mitochondrial membrane is the proposed mechanism for transport limitation for the charged molecule ADP [6-7], biological membranes do not constitute any barrier for oxygen diffusion. Nevertheless, oxygen affinity is significantly reduced in respiration of permeabilized muscle fibers [8]. In the present study we addressed the problem of oxygen limited respiration by comparing the oxygen kinetics in permeabilized muscle fibers, collagenasetreated permeabilized muscle fibers and isolated mitochondria.
• O2k-Network Lab: AT Innsbruck Gnaiger E
Labels: MiParea: Respiration, Instruments;methods
Organism: Rat
Tissue;cell: Heart, Skeletal muscle
Preparation: Permeabilized tissue, Isolated mitochondria
Regulation: ADP, O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: LEAK, OXPHOS
HRR: Oxygraph-2k