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Difference between revisions of "Kunz 1993 FEBS Lett"

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Revision as of 07:54, 2 July 2013

Publications in the MiPMap
Kunz WS, Kuznetsov AV, Gellerich FN (1993) Mitochondrial oxidative phosphorylation in saponin-skinned human muscle fibers is stimulated by caffeine. FEBS Let 323: 188-190.

Β» PMID: 8495738

Kunz WS, Kuznetsov AV, Gellerich FN (1993) FEBS Let

Abstract: The addition of 15 mM caffeine to saponin-skinned human muscle fibers from M. vastus lateralis caused in the presence of 2 mM ATP an approx. 2-fold stimulation of respiration with glutamate + malate. This effect can be abolished by either the addition of the Ca2+ chelator EGTA, the inhibitor of Ca2+ transport Ruthenium red and the inhibitor of the myosin ATPase vanadate. The caffeine concentration dependency of respiration of fibers coincided with the caffeine-caused stimulation of myosin ATPase activity. The activation of oxidative phosphorylation in saponin-skinned human muscle fibers by caffeine can be explained by a stimulation of myosin ATPase caused by Ca2+ release from sarcoplasmic reticulum. β€’ Keywords: Saponin-skinned muscle fiber, Human skeletal muscle, Oxidative phosphorylation, Caffeine


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Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: OXPHOS 

HRR: Oxygraph-2k