Kunz 1993 FEBS Lett

Kunz WS, Kuznetsov AV, Gellerich FN (1993) Mitochondrial oxidative phosphorylation in saponin-skinned human muscle fibers is stimulated by caffeine. FEBS Let 323: 188-190.

» PMID: 8495738

Kunz WS, Kuznetsov AV, Gellerich FN (1993) FEBS Let

Abstract: The addition of 15 mM caffeine to saponin-skinned human muscle fibers from M. vastus lateralis caused in the presence of 2 mM ATP an approx. 2-fold stimulation of respiration with glutamate + malate. This effect can be abolished by either the addition of the Ca²⁺ chelator EGTA, the inhibitor of Ca²⁺ transport Ruthenium red and the inhibitor of the myosin ATPase vanadate. The caffeine concentration dependency of respiration of fibers coincided with the caffeine-caused stimulation of myosin ATPase activity. The activation of oxidative phosphorylation in saponin-skinned human muscle fibers by caffeine can be explained by a stimulation of myosin ATPase caused by Ca²⁺ release from sarcoplasmic reticulum. • Keywords: Saponin-skinned muscle fiber, Human skeletal muscle, Oxidative phosphorylation, Caffeine

Labels:

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: OXPHOS 

HRR: Oxygraph-2k