Difference between revisions of "Kulawiak 2008 Exp Neurol"
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{{Publication | {{Publication | ||
|title=Kulawiak B, Kudin AP, Szewczyk A, Kunz WS (2008) BK channel openers inhibit ROS production of isolated rat brain mitochondria. Exp | |title=Kulawiak B, Kudin AP, Szewczyk A, Kunz WS (2008) BK channel openers inhibit ROS production of isolated rat brain mitochondria. Exp Neurol 212:543-7. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/18572168 PMID: 18572168 ] | |||
|authors=Kulawiak B, Kudin AP, Szewczyk A, Kunz WS | |authors=Kulawiak B, Kudin AP, Szewczyk A, Kunz WS | ||
|year=2008 | |year=2008 | ||
|journal= | |journal=Exp Neurol | ||
|abstract=To delineate the potential mechanism of neuroprotective effects of potassium channel openers we have investigated, how Ca<sup>2+</sup>-activated large conductance potassium channel (BKCa channel) openers influence the production of reactive oxygen species (ROS) by rat brain mitochondria, since mitochondrial generation of ROS is known to have a crucial influence on neuronal survival. We studied the effects of BKCa channel openers CGS 7184 and NS 1619 on hydrogen peroxide production rate of isolated rat brain mitochondria. In K<sup>+</sup>-containing media 3 μM of both channel openers reduced the hydrogen peroxide production rates by approximately 20%. This effect was not observed in Na<sup>+</sup>-containing media. This potassium-dependent partial inhibition of hydrogen peroxide production was found to be sensitive to the selective blockers of BKCa channel iberiotoxin and charybdotoxin applied in nanomolar concentrations. Taken together, our data are compatible with the viewpoint that the opening of a Ca<sup>2+</sup-activated large conductance potassium channel being localised in the inner membrane of brain mitochondria inhibits ROS production by respiratory chain complex I. This finding is suggested to explain the beneficial effects of BK potassium channel openers on neuronal survival. | |abstract=To delineate the potential mechanism of neuroprotective effects of potassium channel openers we have investigated, how Ca<sup>2+</sup>-activated large conductance potassium channel (BKCa channel) openers influence the production of reactive oxygen species (ROS) by rat brain mitochondria, since mitochondrial generation of ROS is known to have a crucial influence on neuronal survival. We studied the effects of BKCa channel openers CGS 7184 and NS 1619 on hydrogen peroxide production rate of isolated rat brain mitochondria. In K<sup>+</sup>-containing media 3 μM of both channel openers reduced the hydrogen peroxide production rates by approximately 20%. This effect was not observed in Na<sup>+</sup>-containing media. This potassium-dependent partial inhibition of hydrogen peroxide production was found to be sensitive to the selective blockers of BKCa channel iberiotoxin and charybdotoxin applied in nanomolar concentrations. Taken together, our data are compatible with the viewpoint that the opening of a Ca<sup>2+</sup>-activated large conductance potassium channel being localised in the inner membrane of brain mitochondria inhibits ROS production by respiratory chain complex I. This finding is suggested to explain the beneficial effects of BK potassium channel openers on neuronal survival. | ||
|keywords= BK channel opener, Brain mitochondria, ROS production | |keywords=BK channel opener, Brain mitochondria, ROS production | ||
| | |mipnetlab=PL Warsaw Szewczyk A | ||
|discipline=Mitochondrial Physiology, Biomedicine | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |organism=Rat | ||
| | |tissues=Nervous system | ||
|enzymes=Complex I, Inner mt-membrane transporter | |||
|couplingstates=OXPHOS | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Mitochondrial Physiology, Biomedicine | |||
}} | }} | ||
Latest revision as of 16:39, 17 February 2015
| Kulawiak B, Kudin AP, Szewczyk A, Kunz WS (2008) BK channel openers inhibit ROS production of isolated rat brain mitochondria. Exp Neurol 212:543-7. |
Kulawiak B, Kudin AP, Szewczyk A, Kunz WS (2008) Exp Neurol
Abstract: To delineate the potential mechanism of neuroprotective effects of potassium channel openers we have investigated, how Ca2+-activated large conductance potassium channel (BKCa channel) openers influence the production of reactive oxygen species (ROS) by rat brain mitochondria, since mitochondrial generation of ROS is known to have a crucial influence on neuronal survival. We studied the effects of BKCa channel openers CGS 7184 and NS 1619 on hydrogen peroxide production rate of isolated rat brain mitochondria. In K+-containing media 3 μM of both channel openers reduced the hydrogen peroxide production rates by approximately 20%. This effect was not observed in Na+-containing media. This potassium-dependent partial inhibition of hydrogen peroxide production was found to be sensitive to the selective blockers of BKCa channel iberiotoxin and charybdotoxin applied in nanomolar concentrations. Taken together, our data are compatible with the viewpoint that the opening of a Ca2+-activated large conductance potassium channel being localised in the inner membrane of brain mitochondria inhibits ROS production by respiratory chain complex I. This finding is suggested to explain the beneficial effects of BK potassium channel openers on neuronal survival. • Keywords: BK channel opener, Brain mitochondria, ROS production
• O2k-Network Lab: PL Warsaw Szewczyk A
Labels:
Organism: Rat
Tissue;cell: Nervous system
Enzyme: Complex I, Inner mt-membrane transporter
Coupling state: OXPHOS
HRR: Oxygraph-2k
