Difference between revisions of "Krivakova 2007 Physiol Res"
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{{Publication | {{Publication | ||
|title=Krivakova P, Labajova A, Cervinkova Z, Drahota Z (2007) Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes. Physiol Res 56:137-40. | |title=Krivakova P, Labajova A, Cervinkova Z, Drahota Z (2007) Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes. Physiol Res 56:137-40. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/17381246 PMID: 17381246] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/17381246 PMID: 17381246 Open Access] | ||
|authors= | |authors=Stankova Pavla, Labajova Anna, Cervinkova Zuzana, Drahota Zdenek | ||
|year=2007 | |year=2007 | ||
|journal=Physiol Res | |journal=Physiol Res | ||
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maximum capacity of the succinate oxidase system was inhibited by 50 % in comparison with values obtained in the absence of t-butyl hydroperoxide. Our data thus indicate that the oxygraphic evaluation of mitochondrial function | maximum capacity of the succinate oxidase system was inhibited by 50 % in comparison with values obtained in the absence of t-butyl hydroperoxide. Our data thus indicate that the oxygraphic evaluation of mitochondrial function | ||
represents a useful tool for evaluation of changes participating in peroxidative damage of cell energy metabolism. | represents a useful tool for evaluation of changes participating in peroxidative damage of cell energy metabolism. | ||
|keywords=Hepatocytes, Oxidative phosphorylation, | |keywords=Hepatocytes, Oxidative phosphorylation, t-Butyl hydroperoxide | ||
|mipnetlab= | |mipnetlab=CZ Hradec Kralove Cervinkova Z | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Latest revision as of 11:13, 18 March 2020
| Krivakova P, Labajova A, Cervinkova Z, Drahota Z (2007) Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes. Physiol Res 56:137-40. |
Stankova Pavla, Labajova Anna, Cervinkova Zuzana, Drahota Zdenek (2007) Physiol Res
Abstract: Using high-resolution oxygraphy, we tested the changes of various parameters characterizing the mitochondrial energy provision system that were induced by peroxidative damage. In the presence of succinate as respiratory substrate, 3 mM t-butyl hydroperoxide increased respiration in the absence of ADP, which indicated partial uncoupling of oxidative phosphorylation. Low activity of coupled respiration was still maintained as indicated by the ADP-activated and oligomycin-inhibited respiration. However, during the incubation the phosphorylative capacity decreased as indicated by the continuous decrease of the mitochondrial membrane potential. Under these experimental conditions the maximum capacity of the succinate oxidase system was inhibited by 50 % in comparison with values obtained in the absence of t-butyl hydroperoxide. Our data thus indicate that the oxygraphic evaluation of mitochondrial function represents a useful tool for evaluation of changes participating in peroxidative damage of cell energy metabolism. • Keywords: Hepatocytes, Oxidative phosphorylation, t-Butyl hydroperoxide
• O2k-Network Lab: CZ Hradec Kralove Cervinkova Z
Labels:
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Liver Preparation: Isolated mitochondria
HRR: Oxygraph-2k
