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Difference between revisions of "Krivakova 2007 Physiol Res"

From Bioblast
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represents a useful tool for evaluation of changes participating in peroxidative damage of cell energy metabolism.
represents a useful tool for evaluation of changes participating in peroxidative damage of cell energy metabolism.
|keywords=Hepatocytes, Oxidative phosphorylation,Β  t-Butyl hydroperoxide
|keywords=Hepatocytes, Oxidative phosphorylation,Β  t-Butyl hydroperoxide
|mipnetlab=CZ Hradec Kralove Cervinkova Z
|mipnetlab=CZ_Hradec Kralove_Cervinkova Z
|discipline=Mitochondrial Physiology
|discipline=Mitochondrial Physiology
}}
}}

Revision as of 09:55, 12 August 2011

Publications in the MiPMap
Krivakova P, Labajova A, Cervinkova Z, Drahota Z (2007) Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes. Physiol Res. 56: 137-140.

Β» PMID: 17381246

Krivakova P, Labajova A, Cervinkova Z, Drahota Z (2007) Physiol. Res.

Abstract: Using high-resolution oxygraphy, we tested the changes of various parameters characterizing the mitochondrial energy provision system that were induced by peroxidative damage. In the presence of succinate as respiratory substrate, 3 mM t-butyl hydroperoxide increased respiration in the absence of ADP, which indicated partial uncoupling of oxidative phosphorylation. Low activity of coupled respiration was still maintained as indicated by the ADP-activated and oligomycin-inhibited respiration. However, during the incubation the phosphorylative capacity decreased as indicated by the continuous decrease of the mitochondrial membrane potential. Under these experimental conditions the maximum capacity of the succinate oxidase system was inhibited by 50 % in comparison with values obtained in the absence of t-butyl hydroperoxide. Our data thus indicate that the oxygraphic evaluation of mitochondrial function represents a useful tool for evaluation of changes participating in peroxidative damage of cell energy metabolism. β€’ Keywords: Hepatocytes, Oxidative phosphorylation, t-Butyl hydroperoxide

β€’ O2k-Network Lab: CZ_Hradec Kralove_Cervinkova Z


Labels:


Organism: Rat 


Enzyme: Complex I, Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property., Uncoupler Protein"Uncoupler Protein" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.  Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Ion Homeostasis"Ion Homeostasis" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Oxygraph-2k