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Difference between revisions of "Konrad 2012 Abstract Bioblast"

From Bioblast
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{{Labeling
{{Labeling
|area=Respiration, mt-Structure; fission; fusion, Environmental and comparative MiP
|area=Respiration, mt-Structure; fission; fusion, Comparative MiP; environmental MiP
|taxonomic group=Crustaceans
|taxonomic group=Crustaceans
|ecomodel=Artemia
|ecomodel=Artemia
|preparations=Isolated Mitochondria
|preparations=Isolated Mitochondria
|enzymes=Adenine Nucleotide Translocase
|enzymes=Adenine Nucleotide Translocase
|injuries=Permeability transition, pH, Mitochondrial Disease; Degenerative Disease and Defect
|injuries=Permeability transition, pH
|kinetics=ADP; Pi, Calcium
|kinetics=ADP; Pi, Calcium
|instruments=Oxygraph-2k, Ca, Fluorometry
|instruments=Oxygraph-2k, Fluorometry, Ca
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|articletype=Abstract
|articletype=Abstract

Revision as of 14:06, 10 August 2013

Konrad C, Kiss G, Torocsik B, Adam-Vizi V, Chinopoulos C (2012) Absence of Ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in C. crangon and P. serratus. Mitochondr Physiol Network 17.12.

Link: MiPNet17.12 Bioblast 2012 - Open Access

Konrad C, Kiss G, Torocsik B, Adam-Vizi V, Chinopoulos C (2012)

Event: Bioblast 2012

Csaba Konrad

Mitochondria from the embryos of brine shrimp (Artemia franciscana) do not undergo Ca2+-induced permeability transition in the presence of a profound Ca2+ uptake capacity [1]. Furthermore, this crustacean is the only organism known to exhibit bongkrekateinsensitive mitochondrial adenine nucleotide exchange, prompting the conjecture that refractoriness to bongkrekate and absence of Ca2+-induced permeability transition are somehow related phenomena [2]. Here we report that mitochondria isolated from two other crustaceans, brown shrimp (Crangon crangon) and common prawn (Palaemon serratus) exhibited bongkrekate-sensitive mitochondrial adenine nucleotide transport, but lacked a Ca2+-induced permeability transition. Ca2+ uptake capacity was robust in the absence of adenine nucleotides in both crustaceans, unaffected by either bongkrekate or cyclosporin A. Transmission electron microscopy images of Ca2+-loaded mitochondria showed needle-like formations of electron-dense material strikingly similar to those observed in mitochondria from the hepatopancreas of blue crab (Callinectes sapidus) [3] and the embryos of Artemia franciscana [2]. Alignment analysis of the partial coding sequences of the adenine nucleotide translocase (ANT) expressed in Crangon crangon and Palaemon serratus versus the complete sequence expressed in Artemia franciscana reappraised the possibility of the 208-214 amino acid region for conferring sensitivity to bongkrekate. However, our findings suggest that the ability to undergo Ca2+-induced mitochondrial permeability transition and the sensitivity of adenine nucleotide translocase to bongkrekate are not necessarily related phenomena.

  1. Menze MA, Hutchinson K, Laborde SM, Hand SC (2005) Mitochondrial permeability transition in the crustacean Artemia franciscana: absence of a calcium-regulated pore in the face of profound calcium storage. Am J Physiol Regul Integr Comp Physiol 289: R68-R76. Open Access
  2. Konrad C, Kiss G, Toeroecsik B, Labar JL, Gerencser AA, Mandi M, Adam-Vizi V, Chinopoulos C (2011) A distinct sequence in the adenine nucleotide translocase from Artemia franciscana embryos is associated with insensitivity to bongkrekate and atypical effects of adenine nucleotides on Ca(2+) uptake and sequestration. FEBS J 278: 822–836.
  3. Chen CH, Greenawalt JW, Lehninger AL (1974) Biochemical and ultrastructural aspects of Ca2+ transport by mitochondria of the hepatopancreas of the blue crab Callinectes sapidus. J Cell Biol 61: 301–315. Open Access

β€’ Keywords: Mitochondrial permeability transition, Crustacean, Bongkrekate

β€’ O2k-Network Lab: HU Budapest Chinopoulos C


Labels: MiParea: Respiration, mt-Structure; fission; fusion"mt-Structure; fission; fusion" is not in the list (Respiration, Instruments;methods, mt-Biogenesis;mt-density, mt-Structure;fission;fusion, mt-Membrane, mtDNA;mt-genetics, nDNA;cell genetics, Genetic knockout;overexpression, Comparative MiP;environmental MiP, Gender, ...) of allowed values for the "MiP area" property., Comparative MiP; environmental MiP"Comparative MiP; environmental MiP" is not in the list (Respiration, Instruments;methods, mt-Biogenesis;mt-density, mt-Structure;fission;fusion, mt-Membrane, mtDNA;mt-genetics, nDNA;cell genetics, Genetic knockout;overexpression, Comparative MiP;environmental MiP, Gender, ...) of allowed values for the "MiP area" property. 

Stress:Permeability transition, pH"pH" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. 


Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.  Enzyme: Adenine Nucleotide Translocase"Adenine Nucleotide Translocase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. 


HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Ca 




Affiliations and author contributions

Csaba Konrad (1), Gergely Kiss (1), Beata Torocsik (1), Vera Adam-Vizi (1), Christos Chinopoulos (1)

(1) Department of Medical Biochemistry, Semmelweis University, Hungary; Email: [email protected]

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