Difference between revisions of "Konrad 2012 Abstract Bioblast"
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{{Abstract | {{Abstract | ||
|title=Konrad C (2012) .. | |title=Konrad C (2012) Absence of Ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in C. crangon and P. serratus. Mitochondr Physiol Network 17.12. | ||
|info=[[MiPNet17.12 Bioblast 2012|MiPNet17.12 Bioblast 2012 - Open Access]] | |info=[[MiPNet17.12 Bioblast 2012|MiPNet17.12 Bioblast 2012 - Open Access]] | ||
|authors=Konrad C | |authors=Konrad C | ||
|year=2012 | |year=2012 | ||
|event=[[Bioblast 2012]] | |event=[[Bioblast 2012]] | ||
|abstract=Mitochondria from the embryos of brine shrimp (Artemia franciscana) do not | |||
undergo Ca2+-induced permeability transition in the presence of a profound Ca2+ uptake | |||
capacity. Furthermore, this crustacean is the only organism known to exhibit bongkrekateinsensitive | |||
mitochondrial adenine nucleotide exchange, prompting the conjecture that | |||
refractoriness to bongkrekate and absence of Ca2+-induced permeability transition are | |||
somehow related phenomena. Here we report that mitochondria isolated from two other | |||
crustaceans, brown shrimp (Crangon crangon) and common prawn (Palaemon serratus) | |||
exhibited bongkrekate-sensitive mitochondrial adenine nucleotide transport, but lacked a | |||
Ca2+-induced permeability transition. Ca2+ uptake capacity was robust in the absence of | |||
adenine nucleotides in both crustaceans, unaffected by either bongkrekate or cyclosporin | |||
A. Transmission electron microscopy images of Ca2+-loaded mitochondria showed | |||
needle-like formations of electron-dense material strikingly similar to those observed in | |||
mitochondria from the hepatopancreas of blue crab (Callinectes sapidus) and the embryos | |||
of Artemia franciscana. Alignment analysis of the partial coding sequences of the adenine | |||
nucleotide translocase (ANT) expressed in Crangon crangon and Palaemon serratus | |||
versus the complete sequence expressed in Artemia franciscana reappraised the | |||
possibility of the 208-214 amino acid region for conferring sensitivity to bongkrekate. | |||
However, our findings suggest that the ability to undergo Ca2+-induced mitochondrial | |||
permeability transition and the sensitivity of adenine nucleotide translocase to bongkrekate | |||
are not necessarily related phenomena. | |||
|mipnetlab=HU Budapest Chinopoulos C, | |||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|articletype=Abstract | |articletype=Abstract | ||
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== Affiliations and author contributions == | == Affiliations and author contributions == | ||
Csaba Konrad (1), Gergely Kiss (1), Beata Torocsik (1), Vera Adam-Vizi (1), Christos Chinopoulos (1) | |||
(1) Department of Medical Biochemistry, Semmelweis University, Hungary; Email: [email protected] | |||
== Figure 1 == | |||
Revision as of 09:41, 15 November 2012
| Konrad C (2012) Absence of Ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in C. crangon and P. serratus. Mitochondr Physiol Network 17.12. |
Link: MiPNet17.12 Bioblast 2012 - Open Access
Konrad C (2012)
Event: Bioblast 2012
Mitochondria from the embryos of brine shrimp (Artemia franciscana) do not undergo Ca2+-induced permeability transition in the presence of a profound Ca2+ uptake capacity. Furthermore, this crustacean is the only organism known to exhibit bongkrekateinsensitive mitochondrial adenine nucleotide exchange, prompting the conjecture that refractoriness to bongkrekate and absence of Ca2+-induced permeability transition are somehow related phenomena. Here we report that mitochondria isolated from two other crustaceans, brown shrimp (Crangon crangon) and common prawn (Palaemon serratus) exhibited bongkrekate-sensitive mitochondrial adenine nucleotide transport, but lacked a Ca2+-induced permeability transition. Ca2+ uptake capacity was robust in the absence of adenine nucleotides in both crustaceans, unaffected by either bongkrekate or cyclosporin A. Transmission electron microscopy images of Ca2+-loaded mitochondria showed needle-like formations of electron-dense material strikingly similar to those observed in mitochondria from the hepatopancreas of blue crab (Callinectes sapidus) and the embryos of Artemia franciscana. Alignment analysis of the partial coding sequences of the adenine nucleotide translocase (ANT) expressed in Crangon crangon and Palaemon serratus versus the complete sequence expressed in Artemia franciscana reappraised the possibility of the 208-214 amino acid region for conferring sensitivity to bongkrekate. However, our findings suggest that the ability to undergo Ca2+-induced mitochondrial permeability transition and the sensitivity of adenine nucleotide translocase to bongkrekate are not necessarily related phenomena.
• O2k-Network Lab: HU Budapest Chinopoulos C
Labels:
Affiliations and author contributions
Csaba Konrad (1), Gergely Kiss (1), Beata Torocsik (1), Vera Adam-Vizi (1), Christos Chinopoulos (1)
(1) Department of Medical Biochemistry, Semmelweis University, Hungary; Email: [email protected]
