Konrad 2012 Abstract Bioblast

Konrad C, Kiss G, Torocsik B, Adam-Vizi V, Chinopoulos C (2012) Absence of Ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in C. crangon and P. serratus. Mitochondr Physiol Network 17.12.

Link: MiPNet17.12 Bioblast 2012 - Open Access

Konrad C, Kiss G, Torocsik B, Adam-Vizi V, Chinopoulos C (2012)

Event: Bioblast 2012

Mitochondria from the embryos of brine shrimp (Artemia franciscana) do not undergo Ca²⁺-induced permeability transition in the presence of a profound Ca²⁺ uptake capacity [1]. Furthermore, this crustacean is the only organism known to exhibit bongkrekateinsensitive mitochondrial adenine nucleotide exchange, prompting the conjecture that refractoriness to bongkrekate and absence of Ca²⁺-induced permeability transition are somehow related phenomena [2]. Here we report that mitochondria isolated from two other crustaceans, brown shrimp (Crangon crangon) and common prawn (Palaemon serratus) exhibited bongkrekate-sensitive mitochondrial adenine nucleotide transport, but lacked a Ca²⁺-induced permeability transition. Ca²⁺ uptake capacity was robust in the absence of adenine nucleotides in both crustaceans, unaffected by either bongkrekate or cyclosporin A. Transmission electron microscopy images of Ca²⁺-loaded mitochondria showed needle-like formations of electron-dense material strikingly similar to those observed in mitochondria from the hepatopancreas of blue crab (Callinectes sapidus) [3] and the embryos of Artemia franciscana [2]. Alignment analysis of the partial coding sequences of the adenine nucleotide translocase (ANT) expressed in Crangon crangon and Palaemon serratus versus the complete sequence expressed in Artemia franciscana reappraised the possibility of the 208-214 amino acid region for conferring sensitivity to bongkrekate. However, our findings suggest that the ability to undergo Ca²⁺-induced mitochondrial permeability transition and the sensitivity of adenine nucleotide translocase to bongkrekate are not necessarily related phenomena.

1. Menze MA, Hutchinson K, Laborde SM, Hand SC (2005) Mitochondrial permeability transition in the crustacean Artemia franciscana: absence of a calcium-regulated pore in the face of profound calcium storage. Am J Physiol Regul Integr Comp Physiol 289: R68-R76. Open Access
2. Konrad C, Kiss G, Toeroecsik B, Labar JL, Gerencser AA, Mandi M, Adam-Vizi V, Chinopoulos C (2011) A distinct sequence in the adenine nucleotide translocase from Artemia franciscana embryos is associated with insensitivity to bongkrekate and atypical effects of adenine nucleotides on Ca(2+) uptake and sequestration. FEBS J 278: 822–836.
3. Chen CH, Greenawalt JW, Lehninger AL (1974) Biochemical and ultrastructural aspects of Ca2+ transport by mitochondria of the hepatopancreas of the blue crab Callinectes sapidus. J Cell Biol 61: 301–315. Open Access

• Keywords: Mitochondrial permeability transition, Crustacean, Bongkrekate

• O2k-Network Lab: HU Budapest Chinopoulos C

Affiliations and author contributions

Csaba Konrad (1), Gergely Kiss (1), Beata Torocsik (1), Vera Adam-Vizi (1), Christos Chinopoulos (1)

(1) Department of Medical Biochemistry, Semmelweis University, Hungary; Email: [email protected]

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• MitoPedia: Terms and abbreviations

Labels: MiParea: Respiration, mt-Structure;fission;fusion, Comparative MiP;environmental MiP 

Stress:Permeability transition  Organism: Artemia, Crustaceans 

Preparation: Isolated mitochondria  Enzyme: Adenine nucleotide translocase  Regulation: ADP, ATP, Calcium, pH 

HRR: Oxygraph-2k, O2k-Fluorometer, Ca