Difference between revisions of "Keeney 2009 Hum Gene Ther"

Revision as of 16:44, 17 September 2010

Keeney PM, Quigley CK, Dunham LD, Papageorge CM, Iyer S, Thomas RR, Schwarz KM, Trimmer PA, Khan SM, Portell FR, Bergquist KE, Bennett JP (2009) Mitochondrial gene therapy augments mitochondrial physiology in a Parkinson's disease cell model. Hum. Gene Ther. 20: 897-907.

» PMID: 19374590

Keeney PM, Quigley CK, Dunham LD, Papageorge CM, Iyer S, Thomas RR, Schwarz KM, Trimmer PA, Khan SM, Portell FR, Bergquist KE, Bennett JP (2009) Human Gene Therapy

Abstract: Neurodegeneration in Parkinson’s disease (PD) affects mainly dopaminergic neurons in the substantia nigra, where age-related, increasing percentages of cells lose detectable respiratory activity associated with depletion of intact mitochondrial DNA (mtDNA). Replenishment of mtDNA might improve neuronal bioenergetic function and prevent further cell death. We developed a technology (‘‘ProtoFection’’) that uses recombinant human mitochondrial transcription factor A (TFAM) engineered with an N-terminal protein transduction domain (PTD) followed by the SOD2 mitochondrial localization signal (MLS) to deliver mtDNA cargo to the mitochondria of living cells. MTD–TFAM (MTD¼PTDþMLS¼‘‘mitochondrial transduction domain’’) binds mtDNA and rapidly transports it across plasma membranes to mitochondria. For therapeutic proof-of-principle we tested ProtoFection technology in Parkinson’s disease cybrid cells, using mtDNA generated from commercially available human genomic DNA (gDNA; Roche). Nine to 11 weeks after single exposures to MTD– TFAMþmtDNA complex, PD cybrid cells with impaired respiration and reduced mtDNA genes increased their mtDNA gene copy numbers up to 24-fold, mtDNA-derived RNAs up to 35-fold, TFAM and ETC proteins, cell respiration, and mitochondrial movement velocities. Cybrid cells with no or minimal basal mitochondrial impairments showed reduced or no responses to treatment, suggesting the possibility of therapeutic selectivity. Exposure of PD but not control cybrid cells to MTD–TFAM protein alone or MTD–TFAMþmtDNA complex increased expression of PGC-1α, suggesting activation of mitochondrial biogenesis. ProtoFection technology for mitochondrial gene therapy holds promise for improving bioenergetic function in impaired PD neurons and needs additional development to define its pharmacodynamics and delineate its molecular mechanisms. It also is unclear whether single-donor gDNA for generating mtDNA would be a preferred therapeutic compared with the pooled gDNA used in this study. Introduction

Labels:

Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.

HRR: Oxygraph-2k

@@ Line 4: / Line 4: @@
 |year=2009
 |journal=Human Gene Therapy
-|abstract=Neurodegeneration in Parkinson’s disease (PD) affects mainly dopaminergic neurons in the substantia nigra,
+|abstract=Neurodegeneration in Parkinson’s disease (PD) affects mainly dopaminergic neurons in the substantia nigra, where age-related, increasing percentages of cells lose detectable respiratory activity associated with depletion of intact mitochondrial DNA (mtDNA). Replenishment of mtDNA might improve neuronal bioenergetic function and prevent further cell death. We developed a technology (‘‘ProtoFection’’) that uses recombinant human mitochondrial transcription factor A (TFAM) engineered with an N-terminal protein transduction domain (PTD) followed by the SOD2 mitochondrial localization signal (MLS) to deliver mtDNA cargo to the mitochondria of living cells. MTD–TFAM (MTD¼PTDþMLS¼‘‘mitochondrial transduction domain’’) binds mtDNA and rapidly transports it across plasma membranes to mitochondria. For therapeutic proof-of-principle we tested ProtoFection technology in Parkinson’s disease cybrid cells, using mtDNA generated from commercially available human genomic DNA (gDNA; Roche). Nine to 11 weeks after single exposures to MTD– TFAMþmtDNA complex, PD cybrid cells with impaired respiration and reduced mtDNA genes increased their mtDNA gene copy numbers up to 24-fold, mtDNA-derived RNAs up to 35-fold, TFAM and ETC proteins, cell respiration, and mitochondrial movement velocities. Cybrid cells with no or minimal basal mitochondrial impairments showed reduced or no responses to treatment, suggesting the possibility of therapeutic selectivity. Exposure of PD but not control cybrid cells to MTD–TFAM protein alone or MTD–TFAMþmtDNA complex increased expression of PGC-1α, suggesting activation of mitochondrial biogenesis. ProtoFection technology for mitochondrial gene therapy holds promise for improving bioenergetic function in impaired PD neurons and needs additional development to define its pharmacodynamics and delineate its molecular mechanisms. It also is
-where age-related, increasing percentages of cells lose detectable respiratory activity associated with depletion of
+unclear whether single-donor gDNA for generating mtDNA would be a preferred therapeutic compared with the pooled gDNA used in this study.
-intact mitochondrial DNA (mtDNA). Replenishment of mtDNA might improve neuronal bioenergetic function
-and prevent further cell death. We developed a technology (‘‘ProtoFection’’) that uses recombinant human
-mitochondrial transcription factor A (TFAM) engineered with an N-terminal protein transduction domain (PTD)
-followed by the SOD2 mitochondrial localization signal (MLS) to deliver mtDNA cargo to the mitochondria of
-living cells. MTD–TFAM (MTD¼PTDþMLS¼‘‘mitochondrial transduction domain’’) binds mtDNA and
-rapidly transports it across plasma membranes to mitochondria. For therapeutic proof-of-principle we tested
-ProtoFection technology in Parkinson’s disease cybrid cells, using mtDNA generated from commercially
-available human genomic DNA (gDNA; Roche). Nine to 11 weeks after single exposures to MTD–
-TFAMþmtDNA complex, PD cybrid cells with impaired respiration and reduced mtDNA genes increased their
-mtDNA gene copy numbers up to 24-fold, mtDNA-derived RNAs up to 35-fold, TFAM and ETC proteins, cell
-respiration, and mitochondrial movement velocities. Cybrid cells with no or minimal basal mitochondrial impairments
-showed reduced or no responses to treatment, suggesting the possibility of therapeutic selectivity.
-Exposure of PD but not control cybrid cells to MTD–TFAM protein alone or MTD–TFAMþmtDNA complex
-increased expression of PGC-1a, suggesting activation of mitochondrial biogenesis. ProtoFection technology for
-mitochondrial gene therapy holds promise for improving bioenergetic function in impaired PD neurons and
-needs additional development to define its pharmacodynamics and delineate its molecular mechanisms. It also is
-unclear whether single-donor gDNA for generating mtDNA would be a preferred therapeutic compared with
-the pooled gDNA used in this study.
 Introduction
 |info=[http://www.ncbi.nlm.nih.gov/pubmed/19374590 PMID: 19374590]