Kampa 2018 MiPschool Tromso C2

Cardioprotective flavoniods as a natural modulators of mitoBKCa channel.

Link: MitoEAGLE

Kampa RP, Kicinska A, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P (2018)

Event: MiPschool Tromso-Bergen 2018

Potassium channels such as K_ATP, BK_Ca or Kv1.3 have been found in the mitochondrial inner membranes (mtIM) of various cells. It is believed that potassium channels regulate mitochondrial membrane potential, matrix volume, respiration, and Ca²⁺ ion homeostasis. There are hypotheses that mitochondrial BK_Ca (mtBK_Ca) channels play an important role in ischemic preconditioning. It was shown that mitoBK_Ca channels are potential targets for some flavonoids in the anti-ischemic strategies.

Our pervious study showed functional properties of the mtBK_Ca channel in mitochondria of endothelial cells (EA.hy 926). Large conductance (270 pS), voltage dependence, a high open-state probability at positive potentials, sensitivity to Ca²⁺, NS1619 (a BK_Ca channel opener) and paxilline (BK_Ca channel inhibitor) indicate similarity to the mammalian BK_Ca channel. Previously, these channels were reported in glioma, skeletal muscle, brain and cardiac tissues.

In the current study, single channel activity of the mtBK_Ca channel was measured with patch-clamp technique of the mitoplasts isolated from EA.hy 926 endothelial cell line. We have shown data describing regulation of the mtBK_Ca channel by different cardioprotective flavonoids (luteolin, quercitin and cyanidin). Cellular respiration tests were performed outusing the oximeter.

• Bioblast editor: Plangger M

Labels: MiParea: mt-Membrane, Pharmacology;toxicology

Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell

Enzyme: Inner mt-membrane transporter Regulation: Ion;substrate transport

Event: C2, Oral Flavonoids

Affiliations and support

Kampa R(1,2), Kicinska A(3), Kulawiak B(2), Jarmuszkiewicz W(3), Szewczyk A(2), Bednarczyk P(1)

Dept Biophysics, Warsaw Univ Life Sciences
Lab Intracellular Ion Channels, Nencki Inst Experimental Biol; Warsaw
Lab Bioenergetics, Adam Mickiewicz Univ, Poznan; Poland

This study was supported by a grant 2016/21/B/NZ1/02769 from the National Science Centre, Poland.