Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Kampa 2018 MiPschool Tromso C2"

From Bioblast
Β 
(9 intermediate revisions by 3 users not shown)
Line 1: Line 1:
{{Abstract
{{Abstract
|title=[[Image:MiPsocietyLOGO.JPG|left|90px|Mitochondrial Physiology Society|MiPsociety]] Cardioprotective flavoniods as a natural modulators of mitoBKCa channel.
|title=[[File:Kampa Rafal.jpeg|left|90px|Rafal Kampa]] Cardioprotective flavoniods as a natural modulators of mitoBKCa channel.
|info=[[MitoEAGLE]]
|info=[[MitoEAGLE]]
|authors=Kampa R, Kicinska A, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P
|authors=Kampa RP, Kicinska A, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P
|year=2018
|year=2018
|event=MiPschool Tromso-Bergen 2018
|event=MiPschool Tromso-Bergen 2018
|abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MitoEAGLE]]
|abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MitoEAGLE]]
Potassium channels such as K<sub>ATP</sub>, BK<sub>Ca</sub> or Kv1.3 have been found in the inner mitochondrial membranes of various cells. It is believed that potassium channels regulate the mitochondrial membrane potential, matrix volume, respiration, and Ca<sup>2+</sup> ion homeostasis. There are hypotheses that mitochondrial BK<sub>Ca</sub> channels play an important role in ischemic preconditioning. It was also shown that mitoBK<sub>Ca</sub> channels are potential targets for some flavonoids in the anti-ischemic strategies.
[[Potassium channel]]s such as K<sub>ATP</sub>, BK<sub>Ca</sub> or Kv1.3 have been found in the mitochondrial inner membranes (mtIM) of various cells. It is believed that potassium channels regulate mitochondrial membrane potential, matrix volume, respiration, and Ca<sup>2+</sup> ion homeostasis. There are hypotheses that mitochondrial BK<sub>Ca</sub> (mtBK<sub>Ca</sub>) channels play an important role in ischemic preconditioning. It was shown that mitoBK<sub>Ca</sub> channels are potential targets for some flavonoids in the anti-ischemic strategies.


Our pervious study, showed functional properties of the mitoBK<sub>Ca</sub> channel in mitochondria of endothelial cells (EA.hy 926). Large conductance (270 pS), voltage dependence, a high open-state probability at positive potentials, sensitivity to Ca<sup>2+</sup>, NS1619 (a BK<sub>Ca</sub> channel opener) and paxilline (BK<sub>Ca</sub> channel inhibitor) indicate similarity to the mammalian BK<sub>Ca</sub> channel. Previously, these channel was reported in glioma, skeletal muscle, brain and cardiac.
Our pervious study showed functional properties of the mtBK<sub>Ca</sub> channel in mitochondria of endothelial cells (EA.hy 926). Large conductance (270 pS), voltage dependence, a high open-state probability at positive potentials, sensitivity to Ca<sup>2+</sup>, [[NS1619]] (a BK<sub>Ca</sub> channel opener) and [[paxilline]] (BK<sub>Ca</sub> channel inhibitor) indicate similarity to the mammalian BK<sub>Ca</sub> channel. Previously, these channels were reported in glioma, skeletal muscle, brain and cardiac tissues.


In the current study, single channel activity of the mitoBK<sub>Ca</sub> channel was measured with patch-clamp technique of the mitoplasts isolated from EA.hy 926 endothelial cell line. We have shown data describing regulation of the mitoBK<sub>Ca</sub> channel by different cardioprotective flavonoids (luteolin, quercitin and cyanidin). Cellular breathing tests using the oximeter were also carried out.
In the current study, single channel activity of the mtBK<sub>Ca</sub> channel was measured with patch-clamp technique of the [[mitoplast]]s isolated from EA.hy 926 endothelial cell line. We have shown data describing regulation of the mtBK<sub>Ca</sub> channel by different cardioprotective flavonoids (luteolin, quercitin and cyanidin). Cellular respiration tests were performed outusing the oximeter.
|editor=[[Plangger M]],
|editor=[[Plangger M]]
}}
}}
{{Labeling
{{Labeling
|area=mt-Membrane
|area=mt-Membrane, Pharmacology;toxicology
|organism=Human
|organism=Human
|tissues=Endothelial;epithelial;mesothelial cell
|tissues=Endothelial;epithelial;mesothelial cell
|enzymes=Inner mt-membrane transporter
|enzymes=Inner mt-membrane transporter
|topics=Ion;substrate transport
|topics=Ion;substrate transport
|event=C2, Oral
|additional=Flavonoids,
}}
}}
== Affiliations ==
== Affiliations and support ==
Kampa R(1,2), Kicinska A(3), Kulawiak B(2), Jarmuszkiewicz W(3), Szewczyk A(2), Bednarczyk P(1) Β 
::::Kampa R(1,2), Kicinska A(3), Kulawiak B(2), Jarmuszkiewicz W(3), Szewczyk A(2), Bednarczyk P(1) Β 
Β 
::::#Dept Biophysics, Warsaw Univ Life Sciences
::::#Dept Biophysics, Warsaw Univ Life Sciences
::::#Lab Intracellular Ion Channels, Nencki Inst Experimental Biol; Warsaw
::::#Lab Intracellular Ion Channels, Nencki Inst Experimental Biol; Warsaw
::::#Lab Bioenergetics, Adam Mickiewicz Univ, Poznan; Poland Β 
::::#Lab Bioenergetics, Adam Mickiewicz Univ, Poznan; Poland Β 


Β 
:::: This study was supported by a grant 2016/21/B/NZ1/02769 from the National Science Centre, Poland.
== Support ==
This study was supported by a grant 2016/21/B/NZ1/02769 from the National Science Centre, Poland.

Latest revision as of 06:59, 21 February 2020

Rafal Kampa
Cardioprotective flavoniods as a natural modulators of mitoBKCa channel.

Link: MitoEAGLE

Kampa RP, Kicinska A, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P (2018)

Event: MiPschool Tromso-Bergen 2018

COST Action MitoEAGLE

Potassium channels such as KATP, BKCa or Kv1.3 have been found in the mitochondrial inner membranes (mtIM) of various cells. It is believed that potassium channels regulate mitochondrial membrane potential, matrix volume, respiration, and Ca2+ ion homeostasis. There are hypotheses that mitochondrial BKCa (mtBKCa) channels play an important role in ischemic preconditioning. It was shown that mitoBKCa channels are potential targets for some flavonoids in the anti-ischemic strategies.

Our pervious study showed functional properties of the mtBKCa channel in mitochondria of endothelial cells (EA.hy 926). Large conductance (270 pS), voltage dependence, a high open-state probability at positive potentials, sensitivity to Ca2+, NS1619 (a BKCa channel opener) and paxilline (BKCa channel inhibitor) indicate similarity to the mammalian BKCa channel. Previously, these channels were reported in glioma, skeletal muscle, brain and cardiac tissues.

In the current study, single channel activity of the mtBKCa channel was measured with patch-clamp technique of the mitoplasts isolated from EA.hy 926 endothelial cell line. We have shown data describing regulation of the mtBKCa channel by different cardioprotective flavonoids (luteolin, quercitin and cyanidin). Cellular respiration tests were performed outusing the oximeter.


β€’ Bioblast editor: Plangger M


Labels: MiParea: mt-Membrane, Pharmacology;toxicology 


Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell 

Enzyme: Inner mt-membrane transporter  Regulation: Ion;substrate transport 


Event: C2, Oral  Flavonoids 

Affiliations and support

Kampa R(1,2), Kicinska A(3), Kulawiak B(2), Jarmuszkiewicz W(3), Szewczyk A(2), Bednarczyk P(1)
  1. Dept Biophysics, Warsaw Univ Life Sciences
  2. Lab Intracellular Ion Channels, Nencki Inst Experimental Biol; Warsaw
  3. Lab Bioenergetics, Adam Mickiewicz Univ, Poznan; Poland
This study was supported by a grant 2016/21/B/NZ1/02769 from the National Science Centre, Poland.