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Difference between revisions of "Kampa 2018 MiPschool Tromso C2"

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(Created page with "{{Abstract |title=left|90px|Mitochondrial Physiology Society|MiPsociety |info=MitoEAGLE |year=2018 |event=MiPschool Tromso-Bergen 2018 |abstra...")
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{{Abstract
{{Abstract
|title=[[Image:MiPsocietyLOGO.JPG|left|90px|Mitochondrial Physiology Society|MiPsociety]]
|title=[[Image:MiPsocietyLOGO.JPG|left|90px|Mitochondrial Physiology Society|MiPsociety]] Cardioprotective flavoniods as a natural modulators of mitoBKCa channel.
|info=[[MitoEAGLE]]
|info=[[MitoEAGLE]]
|authors=Kampa R, Kicinska A, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P
|year=2018
|year=2018
|event=MiPschool Tromso-Bergen 2018
|event=MiPschool Tromso-Bergen 2018
|abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MitoEAGLE]]
|abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MitoEAGLE]]
Potassium channels such as K<sub>ATP</sub>, BK<sub>Ca</sub> or Kv1.3 have been found in the inner mitochondrial membranes of various cells. It is believed that potassium channels regulate the mitochondrial membrane potential, matrix volume, respiration, and Ca<sup>2+</sup> ion homeostasis. There are hypotheses that mitochondrial BK<sub>Ca</sub> channels play an important role in ischemic preconditioning. It was also shown that mitoBK<sub>Ca</sub> channels are potential targets for some flavonoids in the anti-ischemic strategies.
Our pervious study, showed functional properties of the mitoBK<sub>Ca</sub> channel in mitochondria of endothelial cells (EA.hy 926). Large conductance (270 pS), voltage dependence, a high open-state probability at positive potentials, sensitivity to Ca<sup>2+</sup>, NS1619 (a BK<sub>Ca</sub> channel opener) and paxilline (BK<sub>Ca</sub> channel inhibitor) indicate similarity to the mammalian BK<sub>Ca</sub> channel. Previously, these channel was reported in glioma, skeletal muscle, brain and cardiac.
In the current study, single channel activity of the mitoBK<sub>Ca</sub> channel was measured with patch-clamp technique of the mitoplasts isolated from EA.hy 926 endothelial cell line. We have shown data describing regulation of the mitoBK<sub>Ca</sub> channel by different cardioprotective flavonoids (luteolin, quercitin and cyanidin). Cellular breathing tests using the oximeter were also carried out.
|editor=[[Plangger M]],
|editor=[[Plangger M]],
}}
}}
{{Labeling}}
{{Labeling
|area=mt-Membrane
|organism=Human
|tissues=Endothelial;epithelial;mesothelial cell
|enzymes=Inner mt-membrane transporter
|topics=Ion;substrate transport
}}
== Affiliations ==
== Affiliations ==
Kampa R(1,2), Kicinska A(3), Kulawiak B(2), Jarmuszkiewicz W(3), Szewczyk A(2), Bednarczyk P(1)
::::#Dept Biophysics, Warsaw Univ Life Sciences
::::#Lab Intracellular Ion Channels, Nencki Inst Experimental Biol; Warsaw
::::#Lab Bioenergetics, Adam Mickiewicz Univ, Poznan; Poland




== References ==
== Support ==
This study was supported by a grant 2016/21/B/NZ1/02769 from the National Science Centre, Poland.

Revision as of 13:59, 5 September 2018

MiPsociety
Cardioprotective flavoniods as a natural modulators of mitoBKCa channel.

Link: MitoEAGLE

Kampa R, Kicinska A, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P (2018)

Event: MiPschool Tromso-Bergen 2018

COST Action MitoEAGLE

Potassium channels such as KATP, BKCa or Kv1.3 have been found in the inner mitochondrial membranes of various cells. It is believed that potassium channels regulate the mitochondrial membrane potential, matrix volume, respiration, and Ca2+ ion homeostasis. There are hypotheses that mitochondrial BKCa channels play an important role in ischemic preconditioning. It was also shown that mitoBKCa channels are potential targets for some flavonoids in the anti-ischemic strategies.

Our pervious study, showed functional properties of the mitoBKCa channel in mitochondria of endothelial cells (EA.hy 926). Large conductance (270 pS), voltage dependence, a high open-state probability at positive potentials, sensitivity to Ca2+, NS1619 (a BKCa channel opener) and paxilline (BKCa channel inhibitor) indicate similarity to the mammalian BKCa channel. Previously, these channel was reported in glioma, skeletal muscle, brain and cardiac.

In the current study, single channel activity of the mitoBKCa channel was measured with patch-clamp technique of the mitoplasts isolated from EA.hy 926 endothelial cell line. We have shown data describing regulation of the mitoBKCa channel by different cardioprotective flavonoids (luteolin, quercitin and cyanidin). Cellular breathing tests using the oximeter were also carried out.


β€’ Bioblast editor: Plangger M


Labels: MiParea: mt-Membrane 


Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell 

Enzyme: Inner mt-membrane transporter  Regulation: Ion;substrate transport 




Affiliations

Kampa R(1,2), Kicinska A(3), Kulawiak B(2), Jarmuszkiewicz W(3), Szewczyk A(2), Bednarczyk P(1)

  1. Dept Biophysics, Warsaw Univ Life Sciences
  2. Lab Intracellular Ion Channels, Nencki Inst Experimental Biol; Warsaw
  3. Lab Bioenergetics, Adam Mickiewicz Univ, Poznan; Poland


Support

This study was supported by a grant 2016/21/B/NZ1/02769 from the National Science Centre, Poland.