View source for Johansen 2011 Acta Physiol Scand

{{Publication
|title=Johansen D, Sanden E, Hagve M, Chu X, Sundset R, Ytrehus K (2011) Heptanol triggers cardioprotection via mitochondrial mechanisms and mitochondrial potassium channel opening in rat hearts. Acta Physiol Scand 201:435-44.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/21070611 PMID: 21070611 ]
|authors=Johansen D, Sanden E, Hagve M, Chu X, Sundset R, Ytrehus K
|year=2011
|journal=Acta Physiol Scand
|abstract='''Aim:''' To investigate mechanisms behind heptanol (Hp)-induced infarct size reduction and in particular if protection by pre-treatment with Hp is triggered through mitochondrial mechanisms.

'''Methods:''' Langendorff perfused rat hearts, isolated mitochondria and isolated myocytes were used. Infarct size, mitochondrial respiration, time to mitochondrial permeability transition pore (MPTP) opening and AKT and glycogen synthase kinase 3 beta (GSK-3β) phosphorylation were examined.

'''Results:''' Pre-treatment with Hp reduced infarct size from 29.7 ± 3.4% to 12.6 ± 2.1%. Mitochondrial potassium channel blockers 5-hydroxy decanoic acid (5HD) blocking mitoK(ATP) and paxilline (PAX) blocking mitoK(Ca) abolished cardioprotective effect of Hp (Hp + 5HD 36.7 ± 2.9% and Hp + PAX 40.2 ± 2.8%). Hp significantly reduced respiratory control ratio in both subsarcolemmal and interfibrillar mitochondria in a dose-dependent manner (0.5-5.0 mm). The ADP oxygen ratio was also significantly reduced by Hp (2 mm). Laser scanning confocal microscopy of tetramethylrhodamine-loaded isolated rat myocytes using line scan mode showed that Hp increased time to MPTP opening. Western blot analysis showed that pre-treatment with Hp increased phosphorylation of AKT and GSK-3β before ischaemia and after 30 min of global ischaemia.

'''Conclusion''': Pre-treatment with Hp protects the heart against ischaemia-reperfusion injury. This protection is most likely mediated via mitochondrial mechanisms which initiate a signalling cascade that converges on inhibition of opening of MPTP.
|keywords=Cardioprotection, Gap junction, Heptanol, Ischaemia, Mitochondria, Potassium channels
|mipnetlab=NO Tromsoe Larsen TS
}}
{{Labeling
|organism=Rat
|tissues=Heart
|preparations=Isolated mitochondria
|injuries=Ischemia-reperfusion
|instruments=Oxygraph-2k
}}