Jaburek 2013 Int J Biochem Cell Biol

Jaburek M, Jezek J, Zelenka J, Jezek P. (2013) Antioxidant activity by a synergy of redox-sensitive mitochondrial phospholipase A2 and uncoupling protein-2 in lung and spleen. Int J Biochem Cell Biol 45:816-25

» PMID: 23354121

Jaburek M, Jezek J, Zelenka J, Jezek P (2013) Int J Biochem Cell Biol

Abstract: Mitochondrial uncoupling protein-2 (UCP2) has been suggested to participate in the attenuation of the reactive oxygen species production, but the mechanism of action and the physiological significance of UCP2 activity remain controversial. Here we tested the hypothesis that UCP2 provides feedback downregulation of oxidative stress in vivo via synergy with an H₂O₂-activated mitochondrial calcium-independent phospholipase A₂ (mt-iPLA₂). Tert-butylhydroperoxide or H₂O₂ induced free fatty acid release from mitochondrial membranes as detected by gas chromatography/mass spectrometry, which was inhibited by r-bromoenol lactone (r-BEL) but not by its stereoisomer s-BEL, suggesting participation of mt-iPLA₂γ isoform. Tert-butylhydroperoxide or H₂O₂ also induced increase in respiration and decrease in mitochondrial membrane potential in lung and spleen mitochondria from control but not UCP2-knockout mice. These data suggest that mt-iPLA₂γ-dependent release of free fatty acids promotes UCP2-dependent uncoupling. Upon such uncoupling, mitochondrial superoxide formation decreased instantly also in the s-BEL presence, but not when mt-iPLA₂ was blocked by R-BEL and not in mitochondria from UCP2-knockout mice. Mt-iPLA₂γ was alternatively activated by H₂O₂ produced probably in conjunction with the electron-transferring flavoprotein:ubiquinone oxidoreductase (ETFQOR), acting in fatty acid β-oxidation. Palmitoyl-d,l-carnitine addition to mouse lung mitochondria, respiring with succinate plus rotenone, caused a respiration increase that was sensitive to r-BEL and insensitive to s-BEL. We thus demonstrate for the first time that UCP2, functional due to fatty acids released by redox-activated mt-iPLA₂γ, suppresses mitochondrial superoxide production by its uncoupling action. In conclusion, H₂O₂-activated mt-iPLA₂γ and UCP2 act in concert to protect against oxidative stress. • Keywords: Lung, Spleen, Mitochondrial uncoupling protein-2 (UCP2), Calcium-independent phospholipase A₂ (mt-iPLA₂), Electron-transferring flavoprotein:ubiquinone oxidoreductase

• O2k-Network Lab: CZ Prague Jezek P

Labels:

Stress:Oxidative stress;RONS  Organism: Mouse 

Preparation: Isolated mitochondria  Enzyme: Complex II;succinate dehydrogenase, Uncoupling protein  Regulation: Fatty acid 

HRR: Oxygraph-2k